NoAge/SexLocalizationCause of immuno- supression Pathological findingsFungi appearance Fungi typeEvolution 142/FForearmCorticotherapy for LEGiant-cell inflammation with polymorphous inflammatory cells and suppurative and necrotic areas Spores of 5-15µ & spherules with endosporulation CoccidioidossisGood 262/FPeriocularDiabetes mellitusSuppurative necrosis; important polymorphous inflammatory infiltrate; impressive vascular invasion Broad, thin-walled, hyaline, aseptate hyphae; irregular branching MucormycosisSepsis, death 333/MForearmNone, after DSCM diagnosis, he tested positive for HIV Dermal inflammatory infiltrate with numerous histiocytes with foamy cytoplasms Small, mildly pleomorphic yeasts with narrow-based buds, melanin +; no hyphae CriptoccocosisDifficult 464/FPretibialCorticotherapy for RPGranulomatous inflammatory infiltrate with suppurative foci Short, septated, dichotomously branched hyphae, rare yeasts Aspergillosis (second biopsy) Very good 556/MArmPostirradiationIschemic dermal necrosisNumerous septated hiphae and rare yeasts Aspergillus & Candida Difficult Diagnosis of Deep Seated Cutaneous Mycoses (DSCM) – Practical Exemplification of Current State of Art Cristiana Popp *, Sabina Zurac *, Razvan Andrei *, Tiberiu Tebeica*, Florica Staniceanu*, Virginia Chitu*, Cleo Rosculet**, Adrian Streinu- Cercel** *Colentina University Hospital **„Matei Bal” National Institute of Infectious Diseases DSCM are rare lesions occurring in imunosupressed patients, sometimes with critical evolution due to multiple factors including immune status, associated diseases and poor therapy response. That emphasizes the importance of early and accurate diagnosis despite the confounding clinical and histopathological aspects. Keys of diagnosis are the high level of susceptibility and patient’s multidisciplinary approach.

Case 1 Case 2 The fungal elements are rather inconspicuous in HE stain. Very important for diagnosis is the routine examination of at least one fungal stain for each inflammatory cutaneous lesion in immunosupressed patients (PAS, Grochott) HEHE HE PAS Grochott

Case 3 Case 4 Grochott HE Grochott Case 5 HE PAS Grochott HE Grochott PAS HE

Protocols of British Society for Medical Mycology The pathologist is not called to establish the exact type of fungi, the pathological report must include: the presence and absence of yeast forms, the presence and absence of hyphae, whether hyphae are septate or aseptate, presence of melanin, the size of fungi, cellular location any specialised structures Applying these standards in routine examination of inflammatory skin biopsies micotic infection can be identified as cause of inflammation, thus improving management of immunocompromised patients. The appearance of fungi can be sufficient to guide treatment, but the golden standard requires either immunohistochemical confirmation of the specific type or confirmatory cultures. Patients with poor immune status have, usually, a long, difficult evolution, with possible fatal outcome due to systemic dissemination of fungal infection. Bronchopneumonia (case 2) HE