6- Microbial Nutrition & Growth. Bacterial growth.

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Presentation transcript:

6- Microbial Nutrition & Growth

Bacterial growth

electron sourcelithotrophs (inorganic) organotrophs (organic)

CHNOPS

Singlet oxygen Toxic forms of oxygen contains O 2 2- (peroxide anion)

Detoxification enzymes Other antioxidants: carotenoids, glutathione, Vit E & C, etc.

Micro- aerophiles

Nitrogen cycle Fig 26.17

acidophiles basophiles/ alkalinophiles Vibrio Ferroplasma neutrophiles Fig. 2.14

Halophiles obligate tolerant Barophiles Extremophiles

Ecological associations Antagonistic Synergistic Symbiotic Biofilms Quorum sensing

Other kinds of samples: food, water, air, soil, etc.

Streak plate

Pour plate

Spread plate

Inoculating needle & loop

pipet & pipetters

micropipette = micropipet = micropipetter Disposable tip

tubes Petri plate flask containers

For beer

agarbroth agar slant deep agar

(for Nutrient Broth: just leave out the agar)

General medium Selective medium (nutrient agar) (MacConkey agar)

Differential medium (MacConkey agar)

Differential media (newer)

candle jar capnophilics

Anaerobic jarGlove box

Laminar flow hood (for aerobes)

Standard freezer -20 o C Ultra deep freezer -70 to -86 o C Liquid nitrogen -196 o C refrigerator

Lyophilization = freeze-drying Other: dessication, crystallization

binary fission

budding fragmentation

Growth curve

Plate count using serial dilutions on spread plates Colony counter

Cell counter

MPN 26 cells /100 ml (from T6.6) Most Probable Number

dry weight Indirect methods metabolic activity

Turbidity spectrophotometer

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