Gregor 2007 Use GFP-Bcd to further 2005 work Use time-lapse two-photon microscopy – Fast and sensitive See Bcd on nuclei and anterior to posterior gradient

Gregor 2007 Fluorescence increases with time

Quick Summary Bcd pattern seems to be established early and then stay regardless of – Nuclear proliferation – Nuclei size changes – Appearance of cell membranes Rises and falls with mitosis – Stays constant within for different divisions D is really small Propose a new model that specifies nuclear degradation

Castle More recently have shown that characteristic lengths of source ~ gradient length Suggest there might be localized production necessary to give rise to patterns Big focus on methods  take issue with the photobleaching in Gregor 2007 Model their work and find their estimate of D is off and diffusion model still holds

Is GFP-Bcd functional/normal? Yes. GFP-Bcd can rescue a knockout Pattern the same as wt Stain for endogenous and GFP Bcd show colocalization

Getting strange Bcd associated with nuclei seems to spread out during mitosis? Pretty constant

Is it just binding/unbinding from nuclei? Use photobleaching to show recovery – Implies transport across nuclear membrane – And also degredation Make a new model