Downstream processing

Slides:



Advertisements
Similar presentations
Ways to separate mixtures – Chapter 3: Matter & Its Properties
Advertisements

Matter: Properties & Change
Jeopardy Game Matter (SOL 5.4). States of Matter Phases 10 pts 20 pts 30 pts 40 pts 10 pts 20 pts 30 pts 40 pts Solutions/ Mixtures 10 pts 20 pts 30 pts.
Section 12.4: Phase Changes
PHASE CHANGES
Recovery and Purification of Bio-Products (Chapter 11, M
Advanced Bioprocess Engineering Recovery and Purification of Products Lecturer Dr. Kamal E. M. Elkahlout Assistant Prof. of Biotechnology.
Downstream Processing
The various processes used for the actual recovery of useful products from fermentation or any other process together constitute ‘downstream processing’
Close your books and take out a piece of paper. Give an example of each of the following. 1)Element that exist as a solid at room temperature (298.15K)
Separating Mixtures.
Miscellaneous Process By: Dr. Tahseen Ismail By: Dr. Tahseen Ismail.
Separating Mixtures.
Chapter 14 Section 14.1 Types of Mixtures
Matter: Properties & Change
Bioseparation Technology
Topic 17: States of Matter Table of Contents Topic 17 Topic 17 Click box to view movie clip.
Separating Mixtures.
Matter.
Separating Mixtures.
DRYING Dr. Basavaraj K. Nanjwade M. Pharm., Ph. D
States of Matter. There are Four States of Matter Solid Solid Liquid Liquid Gas Gas Plasma Plasma.
Section 3.3 Mixtures of Matter. Composition of Matter Matter SubstanceMixture.
Matter I. States of Matter  Kinetic Molecular Theory  States of Matter.
Matter: Properties and Changes Mixtures What is a mixture? when two or more substances are combined the individual substances retain their individual chemical.
Chapter 7 Lesson 3. Physical Change A change in size, shape, form, or state of matter Matter’s identity stays the same Matter does not become something.
Matter - Properties and Changes Chapter 3. Substances Substance = Matter that has a uniform and unchanging composition Examples are salt and water Is.
MECHANICAL SEPARATIONS. 6/2/20162 Course Outline Introduction to Separation Processes Role of Mechanical Separations in Chemical Engineering Fundamentals.
CHAPTER 6 REVIEW. Boiling Point  The temperature at which a liquid begins to enter the gaseous state.
Mixtures and Solutions Notes  Pure substance: matter that has definite chemical and physical properties  Mixture: matter that contains two or more substances.
SOLIDS. Properties Solid particles have fixed positions Particles are very close together Solids have fixed shapes and fixed volumes Usually exist in.
Matter anything that has mass and takes up space.
Ways to separate mixtures – Chapter 3: Matter & Its Properties
Methods of Separating Mixtures
+ Separation Methods. + Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties.
Chapter 1.3 Properties of Matter. Intensive Properties Independent of the amount of the sample Used for Identification Examples: temperature, melting.
DOWNSTREAM PROCESSING
Classify separating techniques Clara Ayoub & Lara Siklaoui 5 th hour Oct. 19, 2013 Clara Ayoub & Lara Siklaoui 5 th hour Oct. 19, 2013.
PHYSICAL AND CHEMICAL PROPERTIES Sections 2.2 and 2.3.
Bioseparation Engineering Introduction. Biotechnology built on the genetic manipulation of organisms to produce commercial products or processes Biochemical.
Physical vs. Chemical. Physical Property: A Feature of a material that can be observed or measured without changing the composition of the material –Ex:
Matter- Mixtures and their Separation
Physical Properties of Matter. You live in a huge universe of matter. Because you cannot live without a sense of order. Our sorting techniques are usually.
Waste Treatment, Physical
Chemical Properties and Change Chemical Change Elements versus Compounds Chemical Symbols Chemical Reactions Law of Conservation of Mass.
Separating Mixtures.
Matter and Energy Objectives:  Distinguish between a mixture and a pure substance  Describe four common states of matter and how particles behave in.
Quiz Define a mixture. Define a compound.
Matter and Its Properties Chapter E1. Matter and Physical Properties (E6)  All things are made up of __________, which is anything that has mass and.
Phase Changes (Changes of State). CA State Content Standards 3e. Students know that in solids the atoms are closely locked in position and can only vibrate;
The 3 States of Matter. Kinetic Theory : Concepts for “States” of Matter All atoms and molecules are always in Motion Molecules in solids, liquids and.
+ Separation Methods Ways to separate mixtures. + Separating Mixtures Substances in a mixture are physically combined, so processes based on differences.
SEPARATION METHODS FOR MIXTURES. The key is to find a property that one part of the mixture does have but the other doesn't. This relies on their physical.
Chapter 3 Solids, Liquids & Gases
Classifying. States of Matter Definite shape and volume Particles tightly packed together Expands when heated incompressible.
CHEM 9 STATES OF MATTER. anything that has mass & takes up space Matter Mass the amount of matter an object contains.
Hosted by Miss Dell It’s all about the STATE! Gas Laws- they MUST obey What’s the Matter? Properties- Both Physical and Chemical.
Jeopardy S, L, G Properties Phase Changes Heating Curve ChangesMisc Final Jeopardy.
SEPARATION METHODS. Objectives Explain the role of separations operations in industrial chemical process Explain what constitutes the separation of a.
Matter: Properties & Change
Mixtures.
분리정제기술.
Recovery and Purification of Bio-Products
SEDIMENTATION 9/11/2018.
Separating Mixtures.
Separating Mixtures.
The Classification of Matter Unit 3 - Chemistry
Down stream Processing
Separating Mixtures.
Presentation transcript:

Downstream processing P.PRAKASH BTE06 - 025

Downstream processing The various stages of processing that occur after the completion of the fermentation or bioconversion stage, including separation, purification, and packaging of the product

Stages in Downstream Processing Removal of insoluble's Product Isolation Product Purification Product Polishing

Removal of insoluble's capture of the product as a solute in a particulate-free liquid Example separation of cells, cell debris or other particulate matter from fermentation broth containing an antibiotic.

Typical operations Filtration A mechanical operation used for the separation of solids from fluids (liquids or gases) by interposing a medium to fluid flow through which the fluid can pass, but the solids in the fluid are retained.

Filter media two main types of filter media are solid sieve which -traps the solid particles bed of granular materials -retains the solid particles

Points to be considered while selecting the filter media: ability to build the solid. minimum resistance to flow the filtrate. resistance to chemical attack. minimum cost. long life

Centrifugation use of the centrifugal force for the separation of mixtures More-dense components migrate away from the axis of the centrifuge less-dense components migrate towards the axis.

Flocculation process where a solute comes out of solution in the form of flocs or flakes. Particles finer than 0.1 µm in water remain continuously in motion due to electrostatic charge which causes them to repel each other Once their electrostatic charge is neutralized (use of coagulant) the finer particles start to collide and combine together . These larger and heavier particles are called flocs.

Product Isolation reducing the volume of material to be handled and concentrating the product. the unit operations involved -Solvent extraction -ultra filtration -precipitation

Precipitation formation of a solid in a solution during a chemical reaction. solid formed is called the precipitate and the liquid remaining above the solid is called the supernate.

Product Purification To separate contaminants that resemble the product very closely in physical and chemical properties. Expensive and require sensitive and sophisticated equipment.

Crystallization process of formation of solid crystals precipitating from a solution, melt or more rarely deposited directly from a gas. chemical solid-liquid separation technique, in which mass transfer of a solute from the liquid solution to a pure solid crystalline phase occurs.

Product Polishing final processing steps which end with packaging of the product in a form that is stable, easily transportable and convenient. Crystallization, desiccation, lyophilization and spray drying are typical unit operations

lyophilization freezing the material reducing the surrounding pressure and adding enough heat to allow the frozen water in the material to sublime directly from the solid phase to gas.

THANK U