Microscope Basics T. Trimpe 2005 http://sciencespot.net/

Microscope History Father of Microscopy Antony Van Leewenhoek is known as the ___________________. His first microscopes were in use in the 17th century. Robert Hooke is noted as the first person to realize that all living things contained ___________. Zachariah Janssen created the first___________________, which is what we use in class. cells compound microscope

Microscope Safety Always carry the microscope by holding the following parts: 1. 2. Never touch the ___________. This will smudge them allowing you to not see clearly. After the microscope has been on a while, the ___________ will be hot. Be careful as you are putting the microscope away. Arm Base lenses light/lamp

4X to 40X

Microscope Lenses ocular Eyepiece The eyepiece is called the _________________ lens. Magnification of the eyepiece = _____________ Objective Lenses ocular 10x Lens Magnification Coordinates with the color Total Magnification when looking through the eyepiece Low Power Medium Power High Power 10 X 4 = 40X Eyepiece Low Power 4x Red 10 X 10 = 100X Eyepiece Med. Power 10x Yellow 10 X 40 = 400X Eyepiece High Power Blue 40x

Visual Field/ Field of View What is it? It is the circle of light you see when you look into the microscope. We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Visual Field Comparison Low Power High Power Medium Power

Use the words below to fill in the blanks Smaller 1,000 microns increases micron 3,500 microns Objecs that you will study, such as cells, are much smaller than one millimeter. A millimeter can be divided into 1,000 parts. Each part is called a __________. 1 mm = __________ If the visual field measures 3.5 mm, how many microns is that? ____________________ As you increase your magnification, the amount of what you see in the visual field gets __________. The detail of what you see in the visual field ____________. micron 1,000 microns 3.5x 1,000= 3,500 microns smaller increases

Let’s give it a try ... 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it! 4 – When you are done, turn off the microscope and put up the slides you used.