New Flowcell – How Easy? 1

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SenCell 3

Small, robust, easy to handle Stepless variable spacing 4

SenCell - Specifications Working electrode GC 2mm Reference electrode Salt Bridge - blue ISAAC - green HYREF – black 5

Sensitivity – wall jet Flow cell design  A. thin-layer  B. wall-jet  C. porous flow-through 6

Cotrell Equation 7 ECD  Cottrell equation i lim = 1.47 n F (F m ) 1/3 (DA/b) 2/3 C i lim = C spacer thickness A= WE area A b = spacer thickness C = concentration

Variable spacing – more signal Stepless variable spacing Smaller spacing Higher signal 8

Spacing 9

Sensitivity – more signal 100um vs 12 um spacing 10

Sensitivity – more signal Stepless variable spacing Smaller spacing Higher signal Spacing SenCell (µm) S/N of 100 nM standard DA 5-HT

Sensitivity LOD  50 pM DA (0.23 fmol)  65 pM 5-HT (0.25 fmol) 100 pmole/L standards in Hac + Ringer, 5 uL injection 12

Pressure  Higher flow rate due to UHPLC  Smaller spacing down to 10um 13

Sencell Max Pressure Leak tight ring Rating: 25 bar 14

UHPLC compatible High speed analysis Fast response Noise level time (min) [nA] Current DA 5-HT 100 nmole/L standards in Hac, 5 uL injection 15

UHPLC compatible time (min) [nA] Current DA 5-HT 100 nmole/L standards in Hac, 5 uL injection 16

Conclusion SenCell – The most Easy to use flowcell  Wall jet – performance  Variable spacing - more signal  Less noise by improved fluid dynamics  UHPLC compatible 17