Chapter 12 Essential Concepts in Molecular Pathology Companion site for Molecular Pathology Author: William B. Coleman and Gregory J. Tsongalis.

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Chapter 12 Essential Concepts in Molecular Pathology Companion site for Molecular Pathology Author: William B. Coleman and Gregory J. Tsongalis

Companion site for Molecular Pathology Copyright © 2009 by Academic Press. All rights reserved. 2 FIGURE 12.1 Testing for hepatitis C virus. (A) traditional H&E stained section of liver, (B) amplification curve from viral load testing using the Roche Taqman assay, and (C) HCV genotyping using the Siemens LiPA assay.

Companion site for Molecular Pathology Copyright © 2009 by Academic Press. All rights reserved. 3 FIGURE 12.2 Schematic of real time PCR detection of the t(15;17) in AML. Arrows indicate primers used in amplification.

Companion site for Molecular Pathology Copyright © 2009 by Academic Press. All rights reserved. 4 FIGURE 12.3 Cystic fibrosis mutation analysis using the Hologic CFTR Inplex Invader assay. A schematic diagram illustrates the chemistry involved in the Invader assay and the table represents data output from the 43 mutation panel assay.