Photosynthesis Lab Review

Photosynthesis Plants, algae, and some bacteria convert light energy into chemical energy that is stored in sugars Chlorophyll is the primary pigment in chloroplasts

Plant Pigment Chromatography Paper chromotography is used to separate a mixture into its component molecules The different molecules move up the paper at different rates because of differences in: Solubility, molecular mass, and hydrogen bonding with the paper

You separated plant pigments using an organic solvent (acetone)

Analysis of Results Movement of pigment relative to movement of solvent is a constant, Rf (Reference front) Rf = distance pigment moved/distance solvent moved

Photosynthesis—Light Reactions Light energy excites electrons in plant pigments Boosts them to a higher energy state High energy electrons reduce electron acceptors in the thylakoid membrane Energy is captured in NADPH & ATP

DPIP as an Electron Acceptor You measured rate of electron excitation when light hits chlorophyll DPIP is an electron acceptor As DPIP is reduced, it changes from blue to colorless To measure the color change, you used a spectrophotometer Measures amount of light absorbed or transmitted by the sample

Design of the Experiment

Results As chlorophyll’s electrons are exited, they reduce the DPIP, and the sample becomes more and more pale, and the transmittance increases

Cellular Respiration Lab Occurs in every cell of all living things Mitochondria Nutrients (sugars) convert ADP to ATP ATP used for all cellular activities that require energy C6H12O6 + 6O2 --------> 6CO2 + 6H2O + ~38 ATP

Design of the Experiment You measured amount of oxygen consumed by peas using a respirometer

Design of the Experiment

Results  temp =  respiration  germination =  respiration