Ｉｎｔｅｇｒａｔｉｎｇ Ｃｅｌｌｓ into Tissues Sho Momota

CAM(Cell-adhesion molecules) ECM(Extra cellular matrix)

ECM Proteoglycans Collagen fibers Multiadhesive matrix proteins

CAM Cadherins Ig (immunoglobulin) super family Selectins Mucins integrins

Cadherins Ca N-teminus Extra cellular region cell membrane 50-60% of the sequence is identical among different cadherins. C-teminus Cytoplasm

Types of cadherin More than 40 different cadherins are known E-cadherin : Preimplantation embryos, non-neural epethelial tissue P-cadherin : Trophblast N-cadherin : nervous system, lens, cardiac and skeletal muscle

Character of cadherin Homophilic (like bind like) E-cadherins bind to E-cadherins P-cadherins bind to P-cadherins E-cadherins usually not bind to P-cadherins

N-CAMs Group of Ca-independent cell-adhesion proteins Ig super family of CAMs Primarily Homophilic interaction Encoded by a single gene.

Selectin Extravasation of leukocytes Blood Sialyl Lewis-x antigen Extravasation of leukocytes require the successive formation and breakage of cell-cell contacts between leukocytes in the blood and endothelial cells lining the vessels Sugar binding lectin domain P-selectin Endothelial cell

Integrin Heterodimers of  and  subunits Exhibit relatively low affinities for the ligands

Accelerates a formation of clot IIb3 integrin platelet Accelerates a formation of clot Collagen or thrombin Conformational change of IIb3 integrin Fibrinogen platelet Activated platelet

Ｄｅａｄｈｅｓｉｏｎ factors Promote cell migration and can remodel the cell surface Disintegrins ECM RGD sequence

De-adhesion factors CELL CAM ECM Disintegrins

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