Use of the New LACF 590 Commonwealth Ave.

The new facility is split into a Barrier and Non Barrier Facility Both are considered “clean” of rodent pathogens including noro- virus.

Barrier vs Non-Barrier The Barrier is used for the breeding of mouse lines that are not commercially available.

Once a mouse leaves the Barrier it does not come back. The Non-Barrier is used once a mouse is involved in an experiment, surgery, behavioral testing, etc. Mice in the Non-Barrier can leave for surgery, behavioral testing, etc. and return.

Non-Barrier Besides experimental mice, the Non- Barrier also houses rats, ferrets, gerbils, zebra finch birds, and both mouse and rats for ABSL-2. In the Non-Barrier there is also a necropsy room, a surgical suite, cagewash facilities and autoclave facilities.

Necropsy Necropsy is to be used only for terminal procedures. Neither animals nor people can return to an animal room after being in the Necropsy.

Surgical Suite The Surgical Suite consists of 3 rooms. –People and instrument prep room. –Actual surgical room. –Animal prep room, which is also the post op recovery room. –This animal prep/post op recovery room can also be used as a procedures room.

Clean and Dirty Caging There will be a cart outside the cagewash room for the placement of dirty cages. There will be a limited but sufficient amount of clean cages, bottles, tops etc available in each rodent room along with cagecards, colored dots and other materials.

Traffic Flow Once again flow is from the cleanest to the dirtiest. Start with the Barrier Facility which is of limited access. Then the clean side of the Cagewash. Then the Mouse room and the rat room Then the bird room & then the ferret room. The last two rooms to be entered are the ABSL- 2 room, and the gerbil room And finally the dirty side of the cagewash.

PLEASE do not go from the dirtier to the cleaner areas. Once you have been in the Non-Barrier area you CAN NOT go back into the Barrier. Once you have been in the dirty side of the cagewash you should not go back into the animal rooms. Once you have been in Necropsy you can not go into another animal room.

For Entry into hallways –Disposable lab coat –Disposable bonnet –Disposable booties –Disposable gloves –Disposable masks For Entry into animal rooms & handling of animals or animal cages. All of the above, PLUS Disposable arm covers. PPE

Gowning Leave lab coats, jackets etc in the entry area. Put on lab coat, bonnet and mask. Put on booties as you cross the imaginary line into the LACF. Put on gloves after you put on booties.

THANK YOU!!!!