I. Basics Electrophysiology is the study of cell electrical activity resulting from ion channel function.

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Presentation transcript:

I. Basics Electrophysiology is the study of cell electrical activity resulting from ion channel function.

Electrolyte composition of the cell

Fundamental laws  Nernst's equation: E r = RT/F ln [C o /C i ]  Driving force = E m - E r  Ohm's law: V = R x I

A simplified view of cell-cell communication  Ligand-gated channels code for input  Voltage-gated channels code for output

Baseline

 Na leak

GABA A IPSPs

GABA B IPSP

Baseline

Subthreshold AMPA EPSP

Threshold AMPA EPSP

NMDA EPSP

NMDA + GABA A PSPs

II. Methods

Selective antagonism of GABA responses in the cuneate nucleus of the anesthetized rat

Configurations of the patch-clamp technique

Patch-clamp dates

The voltage-clamp technique

III. From channels to behavior The thalamocortical augmented response (AR)

Circuits involved in the AR

Cellular mechanism of the AR

Behavioral component of the AR

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