Epithelium and Junctions VIBS 443/602. EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells 1.Plasma.

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Epithelium and Junctions VIBS 443/602

EM 3: region of basal lamina and connective tissue beneath intestinal epithelial absorptive cells 1.Plasma membrane 2.Basal lamina 3.Connective tissue

EM 17; capillary in the lamina propria of the intestine (duodenum); 13,500x 1.Smooth muscle cell 2.Capillary 3.Endothelial cell

Renin granules in Kidney (PAS)

Ileum, monkey (PAS) 249

Trachea, monkey

Esophagus and trachea, monkey – glands in trachea

Larynx (Gallego's stain)

SPECIALIZATION OF EPITHELIA MAINTAIN EXTENSIVE CONTACTS AMONG CELLS STRUCTURALLY AND FUNCTIONALLY POLARIZED JUNCTIONS ZONULA OCCLUDENS - TIGHT JUNCTION (BELT) ZONULA ADHERENS – ADHERING BELT DESMOSOME (MACULA ADHERENS) - SPOT ATTACHMENT GAP JUNCTIONS - COMMUNICATION

Terminal bars

32409

EM 6b: Region showing zonula occludens, zonula adherents, and macula adherents junctions between cells. 1.Zonula occludens 2.Zonula adherens 3.Desmosome EM 6b

EM 6c

EM 4: Continuity between junctional complex and terminal web 1.Terminal web 2.Interdigitation of plasma membrane 3.Terminal bar

EM 4a: Ridges and grooves of zonula occludens visualized by freeze cleave microscopy 1. Zonula occludens

Inside the cell Outside the cell P = Protoplasmic side E = Ectoplasmic side

EM 4a

EM 2a: Particles and pits of gap junction visualized by freeze cleave microscopy 1. Gap junction

EM 2a

Triad with bile duct Liver & spleen with colloidal carbon, rat 118

EM 2: portion of two cells including gap junction 1.Desmosome 2.Gap junction 3.Rough endoplasmic reticulum

absorptive cells of Ileum, monkey 250

Ileum, monkey (PAS)

argentaffin cells of Ileum, monkey

Large intestine or Colon, monkey

EM 4c: intestinal absorptive cell (super nuclear region); 60,000x 1.Budding RER 2.Coated vesicle 3.Golgi 4.Mitochondria 5.Nucleus 6.Plasma membrane 7.Primary lysosome

Endothelial cells lining small blood vessel lumen with smooth muscle cells and fibrolasts in the vessel wall. 1.Lumen 2.Endothelial cell 3.Fibrolast

EM 10a: Endothelial cells lining capillary with pericyte in the vessel wall. 1.Lumen 2.Endothelial cell 3.Tight junction

Skeletal muscle in spermatic cord UT

UT196 valves in large vein of spermatic cord 195

EM 10f: arteriolar wall; 30,000x 1.Apical caeolae 2.Collagen 3.Dense bodies 4.Elastic fiber 5.Endothelial cell 6.Nuclear pore 7.RER

EM 8h: macrophage in testis; 30,000x. 1.Enlarged basal lamina 2.Heterophagic vacuole 3.Leydig cell cytoplasm 4.Nucleolus 5.Myoid cell

Skin abdomen

Skin, scalp

Skin hand monkey 109

Skin hand monkey

Skin 111

Skin, foot

Vagina

EM 8g: epidermis; 7,000x. Note desmosomal connections between cells 1.Keratinocyte 2.Desmosome 3.Melanocyte

EM 16: surface mucosa of stomach; 13,500x 1.Granules of surface mucous cells 2.Mitochondria 3.Nuclei 4.Lumen

Urinary bladder, monkey 160

Ureter – transitional epithelium and smooth muscle 262

Penis – transitional epithelium and surrounding spongy cavernous of penal urethra 277

Penis - transitional epithelium of penal urethra 277