Perfused Bioreactor with Matrix-Enabled Capillary Scaffold (MECS) Team Members: Allyson Fry Bryan Gorman Jonathan Lin William Wong Advisor: Dr. John P. Wikswo A continuation from (Barnett, Garrett, Harvill, Mayer, McClintock)
Objective Design a tissue engineering scaffold and supporting microfluidic control system to guide the development of a network of capillary tubes in vitro.
Introduction Bioreactor Capillary network Matrix-Enabled Capillary Scaffold (MECS) Perfusion
Motivation Simplified model for disease research Human system Control at many levels Variety of uses
Martins-Green
Bioreactor Design Collagen MECS Supply Network Collagen Supply Network Support Filter
Overall Superstructure
Fractal pattern of vasculature
Matrix-Enabled Capillary Scaffold
Fabrication Strategy 1. Use two-layer photolithography to create mold of ‘egg crate’ design 2. Use soft lithography to create a PDMS stamp 3. Use stamp to deposit a sacrificial layer between two layers of collagen
Overall fluidics
Pumps and LabVIEW
Main Control Program pH sensor settings PID coefficients Graphs Histories of graph values Pump settings pH ranges
Main control program
Project Goals Model a complete microvascular network Support cellular processes Allow evaluation of molecular mechanisms Provide environment for endothelial cell differentiation Facilitate formation of stable tubular structures Support a flow of perfusate
Collaborators Dmitry Markov, PhDResearch advisor in microfabrication Lisa McCawley, PhDResearch advisor in tissue culture Philip SamsonResearch advisor in microfabrication Jason Greene Graduate student working on related bioreactor projects Walter GeorgescuAdvice on interfacing pumps with computer Ron ResisererClean room manager
References Martins-Green M, Li Q, Yao M. A New Generation Organ Culture Arising from Cross-Talk Between Primary Human Cell Types. Unpublished. Nanney, Lillian et al. Unpublished research. Tang MD et al. Fabrication of collagen gels that contain patterned micrometer- scale cavities. Adv. Mater, 2004.