From Fig. 1: Photographs of a finished preparation illuminated with transmitted light. The box in i indicates the areas magnified in ii and iii. (ii) shows.

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From Fig. 1: Photographs of a finished preparation illuminated with transmitted light. The box in i indicates the areas magnified in ii and iii. (ii) shows the bilateral set of slow flexor muscles in segment A3, with the N3s on the right side traced in blue. Arrows mark the location of the left N3s as it projects from the ventral nerve cord to the muscles. iii shows the slow flexor muscles on the right side of ii at higher magnification. Weller et.al. (2015) Journal of Undergraduate Neuroscience Education, in press

From Fig. 4: Double labeling of synaptic vesicles using anti-Synapsin antibody (red, SYNORF) and GABAergic motor axon using anti-GABA antibody (green). Full merged montage of a slow flexor muscle on the left side of segment A2 imaged at 10x. The N3s that innervated the muscle fibers is visible as it crosses the muscle from right to left. The single GABAergic axon is visible in the nerve. Up is anterior, left is lateral. Weller et.al. (2015) Journal of Undergraduate Neuroscience Education, in press

From Fig. 4: Double labeling of synaptic vesicles using anti-Synapsin antibody (red) and GABAergic motor axon using anti-GABA antibody (green). Montaged image from a slow flexor muscle on the left side of segment A2 imaged at 20x. The N3s that innervated the muscle fibers is visible as it crosses the muscle from right to left. The single GABAergic axon is visible in the nerve. Up is anterior, left is lateral. Weller et.al. (2015) Journal of Undergraduate Neuroscience Education, in press

From Fig. 4: Double labeling of synaptic vesicles using anti-Synapsin antibody (red, SYNORF) and GABAergic motor axon using anti-GABA antibody (green). High-resolution images (60x) of fine branches of the nerve showing separately the GABAergic axon (GABA; green), the distribution of synapsin in clusters of synaptic vesicles (SYNORF; red), and the areas of colocalization between anti-GABA and anti-Synapsin (Merged; yellow). Weller et.al. (2015) Journal of Undergraduate Neuroscience Education, in press