（ Ⅱ ）臨床生化（ Ⅱ ） Instrumentational Analysis 賴滄海教授 10-23-2007.

Slides:

Advertisements

Similar presentations

Serological tests (Antigen antibody interactions)

Advertisements

ANTIGEN ANTIBODY REACTION

Enzyme Linked Immunosorbent Assay

Prof. of Clinical Chemistry, Mansoura University.

ELISA Enzyme Linked Immunosorbent Assay. Definitions  Antibodies (also known as immunoglobulins abbreviated Ig) are gamma globulin proteins that are.

AB+AG reactions Detect Identify Quantitate antigen or antibody Disadvantage: Cross reaction -similar or common epitope.

1 Lecture: Forensic Serology - Immunoassays Antibody/Antigen reaction provides the means of generating a measurable result. “Immuno” refers to an immune.

Part Three Basic Test Methods

Immunolabeling Technique

Module Two Labeled Test Methods

Diagnostic Microbiology and Immunology

Introduction to Immunoassays

Immunochemical Methods in the Clinical Laboratory

Enzyme-linked Immunosorbent Assay

Experimental systems in Immunology Sadegh Babashah, PhD Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University.

Principles of Immunology Antigen-Antibody Interactions 4/25/06

09 生醫奈米粒子同材料奈米尺寸的粒子與較大尺寸的粒子，其光學等物理性質相異. Gold nanoparticles The Lycurgus Cup dates from Roman times. The glass appears green in daylight (reflected light),

Immunology LectureRobert J. Boackle, Ph.D. Antigen-Antibody Reactions Specific Objectives: THE STUDENT SHOULD BE ABLE TO 1. Discuss immunoglobulin variability.

Radioimmunoassay & Enzyme Linked Immunosorbent Assay

Antigen antibody reactions

Immunoassays Group 1: Kateryna Dyakiv, Emina Hodzic, Chris Mecinski, Nicholas Anaya, and Andrew Strmic.

Experiment ten To detect the HBsAg with ELISA. ELISA Enzyme linked immunosorbent assay is a normal and simple means to detect the Ag and Ab of HBV and.

CLS 420 Clinical Immunology & Molecular Diagnostics

ABDUALLAH SAUD AL-SHETELY

ELISA Equipment. INTRODUCTION TO ELISA ELISA, or Enzyme-Linked Immunosorbent Assay, are immunological procedures used to determine the presence of antibodies.

Basic Immunologic Procedures

Principles of ELISA. Immunoassay Immunochemical reactions form the basis of a diverse range of sensitive and specific clinical assays. In a typical immunochemical.

Enzyme-Linked Immunosorbent Assay

1 CLINICAL CHEMISTRY CHAPTER 6 IMMUNOASSAYS. 2 Introduction –In the last chapter, we discussed a variety of analytical techniques –In this chapter we’ll.

Endocrine function tests

Applications of Immunochemical Methods in the Clinical Laboratory Roger L. Bertholf, Ph.D. Associate Professor of Pathology University of Florida College.

Quality Control Biochemistry

ELISA (aka Enzyme-Linked Immunosorbent Assay) Professor C. Roth 125:315: BME Measurements and Analysis Laboratory Spring 2003.

Enzyme Linked Immunosorbent Assay (ELISA). ELISA Enzyme Linked Immunosorbent Assay (ELISA) Term Was Coined By Engvall and Pearlmann in 1971 Different.

CLS 1113 Introduction to Clinical Laboratory Practices

- Primary serological tests: (Marker techniques) e.g. Enzyme linked immuonosorbent assay (ELISA) Immuno flurescent antibody technique (IFAT) Radio immuno.

毒物分析授課教師：賴滄海教授 LC-MS of drugs of abuse and related compounds.

ELISA Assay. What Is It? Enzyme immunoassay (EIA) is a test used to detect and quantify specific antigen-eliciting molecules involved in biological processes,

毒物分析免疫檢驗賴滄海教授 Characteristics of the Screening and Confirmation Assays  Screening Assays Easy to Perform (automation) Low cost Sensitivity.

Immunochemical methods

Definitions  Antibodies (also known as immunoglobulins abbreviated Ig) are gamma globulin proteins that are found in blood and are used by the immune.

MLAB 2401: Clinical Chemistry Keri Brophy-Martinez Immunoassays 1.

1 Immunoassay Testing Forensic Toxicology. 2 Introduction Antibody/Antigen reaction provides the means of generating a measurable result. “Immuno” refers.

The Enzyme Linked Immunosorbent Assay (ELISA).. Capture ELISAs Antigen Capture: In this, more specific approach, a capturing Ab is adsorbed onto the solid.

Double immunodiffusion(oucherlony technique) 沉澱線的特征與位置抗原抗體的特異性（ identity ; non-identity ; partial identity) 相互間比例分子大小擴散速度濃度.

Chapter 6 Ag-Ab Interactions: Principles & Applications Nov 2, 2006 Fluorescent Ab staining reveals intracellular Ig.

Chapter 6 Ag-Ab Interactions. Nature of the Ag-Ab interaction Immunological assays.

Objective of this course :  Immunology is the study of the body’s immune system & its function and disorders.  Serology is the studdy of blood serum.

ELISA Nada Mohamed Ahmed , MD, MT (ASCP)i.

液相層析質譜分析 LC-MS Method development and Analyte Identification 授課教師：賴滄海教授授課教師：賴滄海教授

毒物分析免疫檢驗賴滄海教授

طرق التحليل المناعي للكشف عن السموم والمؤثرات العقلية

Antigen – Antibody Reactions

ELISA Enzyme-linked Immunosorbant Assay Detects the presence of minutes quantities of either an antibody or an antigen Important diagnostic tool in many.

（ Ι ）臨床生化（ Ι ） Instrumentation Analysis 賴滄海教授

Serum electrophoresis (SEP) Immunodiffusion ELISA.

ELISA Enzyme Linked Immunosorbent Assay Fariba mazrouei.

What is immunoassay? The term “immunoassay” is a combined term of “immuno” (= antigen-antibody-reaction) and “assay” (= determination of the amount of.

Telephone    Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery.

DON XAVIER N.D GUEST LECTURER ST.ALBERT’S COLLEGE.

ELISA (Enzyme-Linked Immunosorbent Assay)

AFFINITY AND AVIDITY A. Affinity - Antibody affinity is the strength of the reaction between a single antigenic determinant and a single combining site.

Labeled Immunoassays.

به نام خدا. به نام خدا سم‌شناسي و مانيتورينگ دارودرماني.

Immunochemical methods

Enzyme Linked Immuno sorbent Assay

Acta Pathol Microbiol Scand and Clin Lab Med use in PPT for external audience verified via Rightsphere 25Jul

Bioinorganic and Immunoassay Group, Seoul Women’s University

Presentation transcript:

（ Ⅱ ）臨床生化（ Ⅱ ） Instrumentational Analysis 賴滄海教授

Individual Steps In the Analytical Processes Specimen acquisition Specimen identification Specimen delivery to laboratory Specimen preparation Specimen loading and aspiration On-analyzer specimen delivery

Ka = 【 Hp -Ab 】【 Hp 】【 Ab 】 Ka = affinity constant

免疫檢驗的分類方式 Homogeneous Heterogeneous 原理 Competitive Sandwich 標誌物質 RIA FIA EIA CLIA

Examples of IAs Radio immunoassay (RIA) Particle –enhanced turbidimetric inhibition IA (PETINIA) Enzyme-linked Immunosorbent Assays (ELISA) Enzyme multiplied Immunoassay Technique (EMIT) Cloned enzyme donor IA (CEDIA) Microparticle capture enzyme IA (M EIA) Solid phase Fluorescence IA (SPFIA) Particle concentration Fluorescence IA (PCFIA) Fluorescence excitation transfer IA (FETI) Substrate-level Fluorescence IA (FPIA) Dissociation-enhanced lanthanide fluoro imnurco assay (DELFIA)