C R O P S T O I N D U S T R Y WP 2 Tasks: 2-1 to 2-3 Task leader: Dimitra Milioni Crops2Industry “Non-food Crops-to-Industry schemes in EU27”

C R O P S T O I N D U S T R Y Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing Task 2.2: To identify European Genetic Resource Centres Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints Work Package 2: Plant breeding

C R O P S T O I N D U S T R Y Varshney et al., 2010 Spanning the gap between genomics and breeding.

C R O P S T O I N D U S T R Y Task 2.1: To survey the generic tools currently available for the selected non-food crops/species research, breeding production and processing Task 2.2: To identify European Genetic Resource Centres Task 2.3: To capitalize on biotechnology tools and exploit the knowledge of the post genomic era for possible future non-food crops improvement Objectives: To identify current molecular genetic technologies (genomic and biotechnological tools) and suggest their potential applications in a crop-specific manner to address a wide range of breeding constraints Work Package 2: Plant breeding

C R O P S T O I N D U S T R Y European Genetic Resource Centres European Cooperative Crop Genetic Resources Program (ECPGR) EURISCO, a web-based catalogue Centre for Genetic Resources, The Netherlands (CGN) Centre for Plant Genetic Resources Poland, Blonie, Poland Istituto di Genetica Vegetale (Germplasm Research Institute), Italy The Nordic Gene Bank, Alnarp, Sweden The Research Institute of Crop Production, Czech Republic UK Plant Genetic Resources Group, UK Germplasm Resources Information Network (GRIN), USA Germplasm as a starting point

C R O P S T O I N D U S T R Y

Molecular Genomics Resource Centres Not one gene but all the genes European Molecular Biology Laboratory – European Bioinformatics Institute (EMBL-EBI) National Centre for Biotechnology Information (NCBI) DNA Databank in Japan (DDBJ) The Institute for Genome Research (TIGR) PlantGDB Brassica Genome Gateway GRAMENE

C R O P S T O I N D U S T R Y

AH Paterson et al. Nature 457, (2009) doi: /nature07723 Orthologous gene families between sorghum, Arabidopsis, rice and poplar. Whole genome shotgun sequencing project

C R O P S T O I N D U S T R Y AH Paterson et al. Nature 457, (2009) doi: /nature07723 Alignment of sorghum, rice and maize.

C R O P S T O I N D U S T R Y Molecular markers to study genetic diversity in sogrhum germplasms Simple Sequence Repeats (SSR) Inter Simple Sequence Repeats (ISSR) Random Amplified Polymorphic DNA (RAPD) Amplified Fragment Length Polymprphism (AFLP) Diversity Arrays Technology (DArT) Single Feature Polymorphism (SFP) Quantitative Trait Locus (QTL)

C R O P S T O I N D U S T R Y Genetic mapping of QTLs for sugar-related traits in a RIL population of Sorghum bicolor L. Moench Shiringani et al., TAQ (2010); 121(2):32336.

C R O P S T O I N D U S T R Y An Induced Sorghum Mutant Population Section of the leaf blade from BTx623, P5A3, and P7H6 Gallery of selected mutant phenotypes

C R O P S T O I N D U S T R Y Production of transgenic sorghum plants Plant Cell, Tissue and Organ Culture doiI: /s TRANSGENIC SWEET SORGHUM WITH ALTEREDLIGNIN COMPOSITION AND PROCESS OF PREPARATION THEREOF (United StatesPatent Application ) Genetic transformation of Sorghum Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus).

C R O P S T O I N D U S T R Y Thank you for your attention Crops2Industry “Non-food Crops-to-Industry schemes in EU27”