Choosing the Appropriate Camera “Resolving” the Application.

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Presentation transcript:

Choosing the Appropriate Camera “Resolving” the Application

The Appropriate Camera If you know the application AND the product, you will win every time If you know either the application or the product, you may win half the time If don’t know either, you will lose

How do I Select the Correct Product? Guess Pick The Cheapest Pick the Most Expensive Call Scott Monroe “Resolve” the Application

Resolving the Application: Provide the Appropriate Resolution for the Application

SpatialTemporal Intensity Appropriate “Resolution”

Spatial Resolution Spatial

Size of pixels 0.61 N.A. Mag = 2 pixels. ? 100 x 1.3 NA

Spatial Resolution Number of pixels Field of View !!

Temporal Resolution Temporal

What is your “Real Time” How much light do you have in a given time interval? Considerations: Digitizer Readout Rate CCD Architecture Quantum Efficiency Read Noise

Temporal Resolution Region of Interest Frame Transfer Small Array Size Interline

Temporal Resolution e e ICCD

Intensity Resolution Intensity

Dim and Bright sources within the same Image Measure small differences in Intensities Measure small Fluctuations on a large background Dynamic Range

Intensity Resolution electrons GrayValues ,00040,00080,000 Signal Mapping to the ADC

Best of “Both Worlds” SpatialTemporal Intensity High Spatial and Intensity Resolution

Best of “Both Worlds” High Spatial and Intensity Resolution Full Frame Acquisition

Best of “Both Worlds” SpatialTemporal Intensity High Temporal and Intensity Resolution

Best of “Both Worlds” High Temporal and Intensity Resolution Binning: On Chip Charge Combination

Best of “Both Worlds” High Temporal and Intensity Resolution On-Chip Multiplication Gain

Best of “Both Worlds” SpatialTemporal Intensity High Spatial and Temporal Resolution

Best of “Both Worlds” High Spatial and Temporal Resolution ( X, Y ) ( X, t ) “Shoot and Shift” or “Kinetics Mode”

Best of “Both Worlds” High Spatial and Temporal Resolution ( X, Y ) ( x, y, t ) “Virtual Chip Mode”

Biological Products

Microscopy Products Tier 1 –CoolSNAPcf Color and Mono Tier 2 –CoolSNAPES and CoolSNAPHQ Tier 3 –SenSys, Quantix, Cascade Tier 4 –MicroMAX Backthinned, I-PentaMAX

Price Points: 1x 4-6x 2x 3x 8x

Biological Products

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras: CoolSNAP 1.3 Mega Pixels Small Pixels 20MHz Interline Architecture

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras SenSys 1.4 Mega Pixels Small Pixels Full Frame Architecture

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras 512BFT SenSys Frame Transfer Architecture 16-bit 100kHz, 1MHz

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras 512BFT SenSys QT 57 Frame Transfer Architecture 12-bit, 1-3MHz

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras 512BFT SenSys 1024B QT bit 100kHz, 1MHz 1.0 Mega Pixels Large pixels

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras 512BFT SenSys 1024B QT 57 I-PMax Gen 3 Intensifier Frame Transfer Architecture

SpatialTemporal Intensity HQ/es/cf Roper Scientific Cameras 512BFT SenSys 1024B QT 57 I-PMax On-Chip Multiplication Gain Frame Transfer Architecture 512B 16 micron pixels

Where Does Your Application Fit? ? SpatialTemporal Intensity HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B

Where Does Your Application Fit? SpatialTemporal Intensity Cortical Imaging ala Toga HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B ?

Where Does Your Application Fit? SpatialTemporal Intensity Tirf Res/Tirf Fluor HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B ?

Where Does Your Application Fit? SpatialTemporal Intensity Embyonic Chick HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B ?

Where Does Your Application Fit? SpatialTemporal Intensity Single Molecule Fluor HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B ?

Where Does Your Application Fit? SpatialTemporal Intensity Live Cell Fluor HQ/es/cf 512BFT SenSys 1024B QT 57 I-PMax 512B ?