Protein Synthesis. Translation Overview One codon One amino acid.

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Presentation transcript:

Protein Synthesis

Translation Overview One codon One amino acid

Transfer RNA

Ribosome Codon-anticodon matching Peptide bond formation

Transcription/TranslationATGCCGTTTGAGGCCTGA TACGGCAAACTCCGGACT UAGCCGUUUGAGGCCUGA

PAE AAA AUGCCGUUUUAGGCCUGA GGC AminoacylSite Exit Site UAC Peptidyl Site MetPhePro

PAE AAA AUGCCGUUUUAGGCCUGA GGC MetProPhePro

Wobble Only 45 different tRNA molecules exist Pairing of the anticodon with the third base of the codon is less specific

Protein Processing Ribosome type depends on ultimate destination of polypeptide FreeBound Polypeptide to be used in cytosol Polypeptide is headed to membrane or beyond via ER

Protein Processing Signal Peptide Signal Recognition Particle Receptor Protein Polypeptide fed into cisterna Rough ER Cytosol

DNA Function Review 1.What are the three sites in a ribosome? 2.Spliceosome:Intron::_____:_____ 3.How is location of translation related to the eventual use of its product? 4.What is wobble?