Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of.

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Presentation transcript:

Qualitative and Quantitative Differences Between Bone Graft Obtained from the Medullary Canal (with a Reamer/Irrigator/Aspirator) and the Iliac Crest of the Same Patient by H. Claude Sagi, Megan L. Young, Louis Gerstenfeld, Thomas A. Einhorn, and Paul Tornetta J Bone Joint Surg Am Volume 94(23): December 5, 2012 ©2012 by The Journal of Bone and Joint Surgery, Inc.

Fig. 1-A Bone harvesting with use of the Reamer/Irrigator/Aspirator (RIA). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.

Histological assessment of Reamer/Irrigator/Aspirator (RIA) and iliac crest (IC) bone graft materials (hematoxylin and eosin). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.

Figs. 3-A and 3-B Initial comparative assessment of gene expression as indicated by a microarray analysis of samples from six patients (identified as 2 and 12-16). H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.

Identification of biological and molecular pathways that showed statistically significant differences in quantitative gene expression between samples obtained with the Reamer/Irrigator/Aspirator (RIA) and samples obtained from the iliac crest. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.

Comparison of the distribution of biological functions associated with the genes in the three relative expression groupings in Figure 4. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.

Validation of the microarray analysis by comparison with qRT-PCR (quantitative reverse transcription-polymerase chain reaction) measurements. H. Claude Sagi et al. J Bone Joint Surg Am 2012;94: ©2012 by The Journal of Bone and Joint Surgery, Inc.