Kyung Jae Jeong, Ph.D., Claes H. Dohlman, M.D., Ph.D., and Daniel S. Kohane, M.D., Ph.D. Harvard-MIT Health Sciences and Technology, Department of Anesthesiology,

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Presentation transcript:

Kyung Jae Jeong, Ph.D., Claes H. Dohlman, M.D., Ph.D., and Daniel S. Kohane, M.D., Ph.D. Harvard-MIT Health Sciences and Technology, Department of Anesthesiology, Children’s Hospital Boston, Harvard Medical School Massachusetts Eye and Ear Infirmary, Harvard Medical School *The authors have no financial interest in the subject matter of this poster.

Khan et al. (2008)  Cell migration  Cell proliferation with minimal immune response  ECM Production  Adhesion  Good biointegration will also reduce the danger of bacterial/fungal infection.

 Dopamine (or DOPA) is a major component of mussel adhesive protein and has good adhesive properties.  Polydopamine coating can form on virtually all surfaces.  Polydopamine coating can further be used to immobilize biological molecules which contain either thiol or amine groups at a high density.  Chemistry is very simple. Lee et al. Nature (2007) Lee et al. Adv. Materials (2009)

PMMA PMMA-RGD Dopa-PMMA Dopa-PMMA-RGD MTS assay on corneal epithelial cells Fluorescence micrographs of epithelial cells. Nucleus (blue) and actin cytoskeleton (red)

PMMA PMMA-RGD Dopa-PMMADopa-PMMA-RGD MTS assay on keratocytes

 Polydopamine coating does not release dopamine monomer (mass spectrometry)  Minimal contraction of the aortic smooth muscle cells (Calcium Flux Assay).  Not significantly high level of proinflammatory cytokines released from the cells seeded on the polydopamine surface (ELISA, IL-6 and TNF- α). Calcium assay. on human aortic smooth muscle cells (HASMC). Red point corresponds to polydopamine coating. Higher fluorescence response corresponds to higher cell contraction. IL-6 concentration from the cells seeded on various surfaces after 24 hours. TNF- α was not detected from neither cell types.

PMMA PMMA-RGD Dopa-PMMA Dopa-RGD-PMMA

PMMADopa-PMMA A small PMMA cylinder was pressed onto collagen gel over night to see if there is any adhesion at the interface. Collagen Gel PMMA Cylinder

 Polydopamine coating enhanced the proliferation of corneal epithelial cells and keratocytes in vitro.  Polydopamine coating is not proinflammatory and is not expected to cause vascular constriction as its monomer would do.  SEM images show ECM secretion from all surfaces.  Both polydopamine-coated surfaces exhibited firm adhesion to collagen gel.  Polydopamine coating may enhance the biointegration of Boston KPro when applied to the stem of the device.