©2014 Waters Corporation 1 ENHANCED MS WITH THE TURN OF KEY.

Slides:

Advertisements

Similar presentations

Smah Abdelhamid. Its an analytical techniques that couples chromatographic and spectral methods to exploit the advantages of each Chromatography: identify.

Advertisements

Gas Chromatography.

Cruces-Blanco, C., Gamiz-Gracia, L., Garcia-Campana A.M., Applications of Capillary Electrophoresis in Forensic Analytical Chemistry Trends in Analytical.

Gas Chromatography There an be many parts to a gas chromatography system but the basic components include: An injection system. A column (controllable.

Using Skyline to Monitor Long- Term Performance Metrics of High-Resolution Mass Spectrometers J. Will Thompson and M. Arthur Moseley Duke Proteomics Core.

Introducing the EASY-nLC™

The geometry of capillary columns is fairly simple, consisting of length, internal diameter, and stationary phase thickness. Nevertheless, there are endless.

Gas Chromatography. Gas Chromatography Basics Gas Liquid Chromatography (GLC) Gas Solid Chromatography (GSC) Mobile phase does not interact with analyte.

Pn. Suryati Bt. Syafri 2009 High Performance Liquid Chromatography (HPLC)

Issues in the Measurement of Oxytocin Armando J. Mendez Department of Medicine Division of Endocrinology, Diabetes And Metabolism Miller School of Medicine.

Waters Corporation Connecting Data to Decisions John Swallow Principal Engineer Waters Data Products

Institute of Chemical Sciences

High Performance Liquid Chromatography High Performance Liquid Chromatography Chem. 331.

Wilkes University - CHM D Gas Chromatography (or 3D with MS Detector) A powerful separations tool for complex volatile mixtures of heat-stable samples.

Created with MindGenius Business 2005® Performance parameters (Advanced) Theoretical Plate Model Performance parameters (Advanced) Theoretical Plate Model.

Gas Chromatography Carrier Gas Flow Control Injector Port Column Column Oven Detector Recorder The GC system consists of gas supplies for the mobile phase.

High Performance Liquid Chromatography (HPLC) Jeannette Comeau CHEE April 2004.

Q-Exactive LC-MS. TurboFlow technology - Special columns - Complex valving - Extra pump UHPLC -Fast separation -Best separation.

Rapid and Accurate Analysis of Microcystins Using UPLC/MS/MS Yongtao (Bruce) Li, Ph.D. Joshua S. Whitaker Eurofins Eaton Analytical,

Types of Liquid Chromatography I. Ion Exchange Chromatography A. Factors influencing retention B. Suppressed ion exchange II. Partitioning Chromatography.

Varian 920-LC Integrated Analytical HPLC

Three years of experience with backflush and retention time locking on Agilent 7000B GC-MS/MS Hans Ragnar Norli Bioforsk, Plant Health and Plant Protection.

Sample Preparation Strategies for Routine Trace Mixture Analysis by NMR David Detlefsen, Kenneth Ray & Jeffrey Whitney.

DEVELOPMENT OF A RP-HPLC METHOD FOR THE DETERMINATION OF METFORMIN IN HUMAN PLASMA.

Peptide hormones: the sensitivity quest and sensitivity questions. Eduard Rogatsky Albert Einstein College of Medicine Einstein-Montefiore Institute for.

Sales Objections and FAQs 920-LC Analytical Systems.

Intensive General Chemistry Chemical separations II Isabelle Vu Trieu

CAN YOUR HPLC COLUMN DO THIS ?. Type B SILICA TYPE-C SILICA 

10 years of evolution in UHPLC-MS chromatography. Current trends. Eduard Rogatsky Albert Einstein College of Medicine Einstein-Montefiore Institute for.

Analytical Separations

Market Segment Training. AA ~$2,000M* GC ~$1,650M* Biotech ~$7,500M* Liquid ~$4,100M* (growth at ~6% per annum) *based on figures from SDI 2004 All prices.

212-LC Pump Technology Background August Dual Piston Pump Single piston pumps deliver then refill, forcing the use of a very large pulse damper.

An Ultrasensitive LC-MS+NMR Platform Utilizing Segmented-Flow Microcoil NMR, Nanospray ESI, and 4 mm LC Columns Yiqing Lin, Paul Vouros, Jimmy Orjala 1,

Diversity of molecules and the resulting analytical challenges CHAOTIC 24 April 2013.

Stability Indicating Assays for Dose Formulation Stability Testing Teresa Fuller GSK.

Chapter 28 High Performance Liquid Chromatography.

Isotope Labeled Internal Standards in Skyline

LC/MS Chromatography Systems 212-LC. 2 The Complete Solution Varian LC components, pumps, autosamplers and the New MS Workstation SW (V6.6) can be used.

= the matrix for T relative to the standard basis is a basis for R 2. B is the matrix for T relative to To find B, complete:

Selling Restek LC Solutions for Analytical Scale Laboratories Rick Lake Product Line Manager Liquid Chromatography x 2379

Determination of metformin in urine (by Liquid Chromatography LC)

Toxicological Screening of 80 Drugs in Urine Using the High Resolution Exactive LC/MS Orbitrap Mass Spectrometer Coupled to Online Extraction and Turbulent.

What Bottlenecks are Slowing Down Your Lab? Software Solutions for Environmental Testing Charles Yang Strategic Marketing Manager Environmental Testing.

The world leader in serving science Alternate Channels Meeting 12 th June 2007 Tom Whitehouse European Commercial Marketing Manager Small Molecules.

Separation Basics UHPLC_LC MSMS & Column Technology

Introduction to Liquid Phase Mass Spectrometry

Protein Identification using 2D LC/MS/MS based on pH Gradient and Reverse Phase Separation Column Technology Inc.,

EQUIPMENT and METHOD VALIDATION

Evolution GC-MS/MS: Pesticide analysis in canola oil Evolution GC-MS/MS: Pesticide analysis in canola oil Vivian Watts 1, Ingo Christ 1, Mark Misunis 2.

Forensic Toxicology Use Only Analysis of ETG, ETS using the Thermo Scientific Exactive Mass Spectrometer Kent Johnson Fortes lab, Portland Oregon.

이 장 우. 1. Introduction  HPLC-MS/MS methodology achieved its preferred status -Highly selective and effectively eliminated interference -Without.

Plasma Free Metanephrines Analysis using LC-MS/MS with Porous Graphitic Carbon Column Xiang He (Kevin) and Marta Kozak Thermo Fisher Scientific.

Multi-Analyte LC-MS/MS Methods – Best Practice.

Accucore UHPLC & HPLC Columns

LC-MS/MS Analysis of Tetracycline antibiotics in Honey using Advance UHPLC-EVOQ™ SIMA LAB PVT LTD.

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Metabolomics.

HPLC Equipment : Detectors

Instrumental Analysis

The analysis of domoic acid using alkaline UPLC-MS/MS

Presentation Title Jet Steam Proteomics with Automated Sample Prep for High-Throughput Peptide Quantitation in Clinical Research 11/16/2018 Confidentiality.

High Resolution Quantitation of Microcystins with SCIEX X500R QTOF System KC Hyland.

Metabolomics: Preanalytical Variables

High Performance Liquid Chromatography

Chromatography Daheeya Alenazi.

Liquid Chromatography - Method Development and Validation

Levofloxacin structural formula

Damiana Gentili Qualified Person/QU Director 09/05/2019

Presentation transcript:

©2014 Waters Corporation 1 ENHANCED MS WITH THE TURN OF KEY

©2014 Waters Corporation 2 Introducing ionKey/MS What does ionKey/MS™ deliver? 2-40x sensitivity vs. your current MS UPLC ® performance and reliability Standardization and consistency Flexibility

©2014 Waters Corporation 3 In Search of a Step Change in Sensitivity?

©2014 Waters Corporation 4 Maximizing signal Maximizing robustness Xevo ® TQ-S A Step Change in Sensitivity

©2014 Waters Corporation 5 Xevo TQ Xevo TQ-S Enhanced sensitivity >25X increase in peak area >5X increase in signal:noise Relative ion abundance Reserpine (50fg) UPLC/MRM Xevo TQ-S A Step Change in Sensitivity

©2014 Waters Corporation 6 Where Next to Look for Sensitivity?

©2014 Waters Corporation 7 Where to Next? 75µm ID 150µm ID 300µm ID + Very Sensitive Flow rates too low for routine use Very Fast Marginal sensitivity increase 0 Fast and Sensitive Useable routinely

©2014 Waters Corporation 8 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 9 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 10 More Sensitivity ionKey/MS 20X Increase Standard 2.1mm column

©2014 Waters Corporation 11 More Sensitivity ionKey/MSStandard Flow LC-MS ionKey/MS 2 Fold suppression in signal due to Matrix Effects 2.1mm 24 Fold suppression in signal due to Matrix Effects

©2014 Waters Corporation 12 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 13 UPLC Performance You Know

©2014 Waters Corporation 14 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 15 Sensitivity Reproducibility Robustness User Expertise Requirement Applicability to Routine Capillary scale UPLC can be challenging

©2014 Waters Corporation 16 ionKey/MS is Plug and Play ENHANCED MS WITH THE TURN OF KEY

©2014 Waters Corporation 17 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 18 Robust, Reliable Reproducible Retention Time Reproducibility 15 QCs over 500 crashed plasma injections Reproducibility of the same compound over: 1.64% RSD 5 Systems 18 iKeys 5 Users

©2014 Waters Corporation 19 Validated Assay Teriparadite (Forteo ® ) Avg MWT

©2014 Waters Corporation 20 THE FUTURE OF MS THE FUTURE OF MS UPLC PERFORMANCE YOU KNOW UPLC PERFORMANCE YOU KNOW MORE SENSITIVITY MORE SENSITIVITY PLUG AND PLAY PLUG AND PLAY ROBUST REPRODUCIBLE RELIABLE ROBUST REPRODUCIBLE RELIABLE

©2014 Waters Corporation 21 The Future of MS Already over 25 publications and presentations in leading scientific journals and conferences such as: Journal of Chromatography, Bioanalysis Journal, Journal of Proteomics, ASMS, HPLC, EBF CPSA, and more. Already over 25 publications and presentations in leading scientific journals and conferences such as: Journal of Chromatography, Bioanalysis Journal, Journal of Proteomics, ASMS, HPLC, EBF CPSA, and more.

©2014 Waters Corporation 22