INTRODUCTION Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes. Rhizobia invade the roots of legume plants.

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Presentation transcript:

INTRODUCTION Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes. Rhizobia invade the roots of legume plants through root hairs, form effective pink colored nodules in the tap or lateral roots and lives symbiotically inside the nodules to fix nitrogen to plant accessible forms It was estimated that a total of 175 million metric tones of N is fixed per year globally through biological nitrogen fixation involving Legume Rhizobium symbiosis. Rhizobium as component of INM

Rhizobium, a root nodule bacterium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes.It was estimated that a total of 175 million metric tones of N is fixed per year globally through biological nitrogen fixation involving Legume Rhizobium symbiosis.

Curling of root hair Invagination point Infection thread develop Bacteria released Nodular tissue develop HOW RHIZOBIA INFECT LEGUME ?? Rhizobium as component of INM

Selection of legume crops Collection of nodules ISOLATION AND MAINTENANCE OF RHIZOBIUM Rhizobium isolation in media Preservation in slant Purified rhizobial cells Nodulation tests Selection of legume crops Collection of nodules Preservation in slant Selection of legume crops Collection of nodules Rhizobium isolation in media Preservation in slant Selection of legume crops Collection of nodules Purified rhizobial cells Rhizobium isolation in media Preservation in slant Selection of legume crops Collection of nodules Nodulation testsPurified rhizobial cells Rhizobium isolation in media Preservation in slant Selection of legume crops Collection of nodules

CHARACTERISTICS OF RHIZOBIUM Rhizobia invade legume roots through root hairs Form effective pink colored nodules in the roots Lives symbiotically inside the nodules and fix nitrogen Converts atmospheric nitrogen to plant accessible forms Most rhizobia are host specific in nature Rhizobia invade legume roots through root hairs Form effective pink colored nodules in the roots Lives symbiotically inside the nodules and fix nitrogen Converts atmospheric nitrogen to plant accessible forms Most rhizobia are host specific in nature Rhizobium as component of INM

50 – 200 KG NITROGEN PER HECTARE PER CROP Rhizobium as component of INM

Rhizobium Inoculation and Crop Response Inoculation Method Nitrogen Fixation Rhizobium culture Crop Response

Contribution of Rhizobium in biological N fixation Crop Rhizobium species Quantum of N fixed (kg N/ha/yr) Soybean R. japonicum Alfalfa R. meliloti Clover R. trifolii Groundnut R. sp Pea R. leguminosarum Cowpea R. sp Green/ Black gram R. leguminosarum Bengal gram Bradyrhizobium sp Rhizobium as component of INM

LET US SUM UP Rhizobium fix atmospheric nitrogen in symbiotic association with host legumes. It was estimated that a total of 175 million metric tonnes of N is fixed per year through legume-Rhizobium symbiosis. Rhizobium lives symbiotically inside the nodules and fix nitrogen which is converted to plant accessible forms. Contd….. Rhizobium as component of INM

Rhizobia invade the roots of legume plants through root hairs and form effective pink colored nodules in the tap or lateral roots. Most Rhizobia are host specific and nodulate specific legumes. Rhizobium as component of INM LET US SUM UP