Supplementary Figure SU11274 PHA SGX EGF/bFGF HGF SU11274 PHA SGX523 A. B number of plated cells EGF/bFGF HGF number of plated cells SU11274 PHA SGX523 SU11274 PHA SGX Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 ) Proportion of wells w/o sphere (log 10 )

2.08%31.8%25.4% 2.83% 9.00%7.53% Isotype-APC CD133-APC 464T +EGF/bFGF 464T +EGF/bFGF -SU T +EGF/bFGF +SU T +HGF 464T +HGF -SU T +HGF +SU11274 Supplementary Figure 2

GFPABCMergeMerge + DAPI Supplementary Figure 3 Field 1 Field 2 Field 3 GFPABCMergeMerge + DAPI GFPABCMergeMerge + DAPI

mCMV backbone reporter mCMV-TCF/LEF reporter 464T Relative Luciferase Unit Wnt3a (ng/ml) Supplementary Figure 4

Supplementary table 1 Symbol Name Fold increase in 464T MET high/+ cells Fold increase in 578T MET high/+ cells References CD44 CD44 antigen Am J Pathol, 1999, 154(2):515 MET MET protooncogene Cancer Res, 2002, 62(18):5126 PLAUR Plasminogen activator receptor, urokinase-type Proc Natl Acad Sci USA, 1999, 96(4):1603 CCND1 Cyclin D Nature, 1999, 398(6726):422 NRCAM Neuronal cell adhesion molecule Genes Dev, 2002, 16(16)2058 FOSL1 Fos-like antigen Proc Natl Acad Sci USA, 1999, 96(4):1603 JUN v-jun avian sarcoma virus 17 oncogene homolog Proc Natl Acad Sci USA, 1999, 96(4):1603 L1CAM L1 cell adhesion molecule J Cell Biol, 2005, 168(4):633 JAG1 Jagged Proc Natl Acad Sci USA, 2009, 106(4)633 CLDN1 Claudin Oncol Res, 2001, 12(11-12):469 TCF7 Transcription factor, T cell-specific Science, 1999, 285(5435):1923 MYC Oncogene myc Science, 1998, 281(5382):1509 BIRC5 Baculoviral IAP repeat-containing protein 5 (Survivin) Cancer Res, 2001, 61(24)8664 LEF1 Lymphoid enhancer-binding factor J Biol Chem, 2002, 277(36)33398 BMP4 Bone morphogenetic protein Cancer Res, 2002, 62(10)2744 FGF18 Fibroblast growth factor Cancer Res, 2003, 63(19)6116 GAST Gastrin J Clin Invest, 2000, 106(4)533 Expression levels of WNT target genes in MET high/+ cells