Biuret Test (Write in glossary):

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Presentation transcript:

Biuret Test (Write in glossary): A simple biochemical reaction to detect the presence of protein. A purple colour shows that protein is present, a blue colour shows that protein is absent.

Protein Assay Purpose and goals: To pipette accurately To plot results To analyse results

Serial Dilution Water (cm3) Protein (cm3) Absorbance (%) 5 1 4 2 3

Quick Questions What was the independent variable? What was the dependent variable? What were the control variables?

Quick Questions Independent variable = conc. of protein Dependent variable = absorbance Control variables = drops of biuret A B, total volume of solution, time