Graham, North Carolina

History of Biotechnology in North Carolina  Significant Events  1958 – established the Research Triangle Park  UNC, NCSU and Duke University  1984 – NC Biotechnology Center  Quasi-governmental agency to integrate industry, academia and financial interests  1985 – Alamance Community College (aka, TCA)  2 year program in Biotechnology Workforce training  2005 – Ernst and Young report  North Carolina 3 rd in nation in Biotechnology  Due to Biomanufacturing industry

Alamance Community College Originally (1985), R&D focus w/ general lab skills, few “biotech” specific Cell Culture Chromatography Immune reactions etc 1993 = added first bioreactors Meet growing industry needs 1L bench top units for bacterial growth Tangential Flow Filtration

Alamance Community College 2005 = 15L CIP/SIP bioreactor Dissolved Oxygen Bacterial and mammalian cell growth Steam generator Computer controls Basic engineering with valves and gauges

Alamance Community College 2006 = FPLC Fast Protein Liquid Chromatography Large volume protein purification To accommodate larger bioreactor SEC, IEC, HIC, AC

Alamance Community College new A+ 1 – 7 liter bioreactors Mammalian & bacterial cell capable Fully computerized