Sampling Methods

Sampling Purpose –Detect and quantify –Identify sources/amplification –Assess potential virulence –Assess effectiveness of controls

Cladosporium

Steps in Sampling Selection of a laboratory Selection of analyts Selection of methods Sensitivity of method Field sampling QC Reporting and lab support

Approaches to Sampling Qualitative - Type Quantitative - Concentration

Overview of Microbial Sample Collection Methods (Liquid Impinger)

Overview of Microbial Sample Collection Methods (Fungal Spore Sampling & Spore Traps)

Overview of Microbial Sample Collection Methods (Impactor Samplers)

Overview of Microbial Sample Collection Methods (Sedimentation Settling Plates)

Overview of Microbial Sample Collection Methods (Airborne Particulates & Bulk Samples)

Overview of Microbial Sample Collection Methods (Dust & Tape Lifts)

Tape Lift Sampling

Overview of Microbial Sample Collection Methods (Swab/Wipe & Surface Contact Plate)

Overview of Microbial Sample Collection Methods (Water Sample & Endotoxins in air)

Overview of Microbial Sample Collection Methods (Mycotoxins in Air)

Overview of Microbial Sample Collection Methods (MVOC’s in Air & β-1,3-D-Glucans in Air)

Microbial By Products (Ergosterol in Air) Notes: *Can also analyze for in bulk, surface and water samples. CFU-colony forming units M3-cubic metres of air Mg-milligrams Ng-nanograms Ppm-parts per million Ml-milliliter G-gram **References used to general Table 1 above are listed below 1-8

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Air Pump Rotameter Biosis Flow Valve Plate Sampling

Stacked Plate Samplers

Impinger Sampling Impingement –Liquid media

Impinger Sampling Types of Impingers –AGI-30 –AGI-4

Impinger Sampling

Air Sampling Types of Filtration –Membrane filter cassette

Passive Sampling Deposition –Gravity on agar

Passive Sampling Types of gravitational –Settling plates

Air Sampling (Spore Trap) Collects “everything” –Viable,non-viable,pollen, Unable to differentiate

Carpet Sampling Collect spores in the carpet pile using air pump Identifies type of mold Looking for uncommon types Provides historical information

Carpet Sampling

Data Interpretation Concentration inside versus outside. Mold types inside versus outside. Presence or absence of a specific mold. Location of unknown source. Change in concentration following remediation. Is a “snap shot” in time.

Stachybotris

Aspergillus

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References