226 PHT Final Spots. Gram’s stain Results: Shape: Cocci Arrangment: irregular clusters Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism:

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Presentation transcript:

226 PHT Final Spots

Gram’s stain

Results: Shape: Cocci Arrangment: irregular clusters Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism: Staphylococci

Results: Shape: Oval Arrangment: Single Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism: Candida

Results: Shape: Bacilli Arrangment: Chains Colour: Violet Gram’s reaction: Gram’s +ve Name of microorganism: Bacillus

Results: Shape: Rods Arrangment: Single Colour: red Gram’s reaction: Gram’s –ve Name of microorganism: Gram negative bacilli Gram negative bacilli

Negative staining Candida

Negative staining Staphylococci

Negative staining of Bacillus

Results Type of Staining: Acid fast stain Shape: beaded bacilli Arrangement: Tree shaped Colour: red Name of microorganism: M.phelei

Spore Stain of Bacillus subtilis Type of Staining: Spore stain Shape: bacilli Arrangement: Chains Colour of spores: green Colour of vegetative cells: red Name of microorganism: B. subtilis

Nutrient agar plate Nutrient agar slant Nutrient broth Simple media

Enriched media Serum agar blood agar

non-hemolytic Sterptococci. β -hemolytic Sterptococci. α -hemolytic Sterptococci. Hemolysis of blood agar

Selective medium L-J

Selective medium Cetremide agar of pseudomonas

 Growth with yellow colour around the coloniesS.aureus  Growth without change in the colour of the mediumS.epidermidis 1-Mannitol salt agar Selective and differential media:

Lactose fermenter Pink colonies Lactose non-fermenterPale colonies 2-MacConkey agar

Cooked meat

Anaerobic Jar

Results: Positive test: formation of visible clot. Coagulase +ve S.aureus Coagulase -ve S.epidermidis 1- Coagulase Test:

2-Oxidase test: Results: +ve Test: Appearance of purple colour within 1-2 min. purple colour +ve test No colour -ve test

Results: +ve-ve 3- Urease Test:

1. Sugar fermentation: GlucoseMaltose Sucrose GlucoseMaltose Sucrose Sugar fermentation can be indicated by change of color of the medium from red to yellow. +ve - ve +ve+ve

Results: Butt: Slant: H 2 S Production: acidic (yellow) -ve acidic (yellow) alkaline (red) -ve acidic (yellow) alkaline (red) +ve 2- Triple Sugar Iron (TSI) agar:

O - /F - O + /F + O + /F - O - /F + Results: Fermentative Oxidative Non Saccharolytic Positive Test: 3-O/F Test (Oxidation Fermentation Test)

Positive Test: 4-Sensitive Oxidation Fermentation (O/F)Test: O - /F - O + /F + O + /F - O - /F + Results: Fermentative Oxidative Non Saccharolytic

Results: +ve -ve 1- Indole test

Methods for determination of MIC

E-test:-

Elek’s Toxigenicity Test: