T. Sampath Kumar Inventor and CEO Nualgi Nanobiotech Nanotechnology solutions Cleaning Rivers rapidly and ecologically using Nualgi T. Sampath Kumar Inventor and CEO Nualgi Nanobiotech

Agriculture Sources Pollution Of activities Human Processing Food Industrial wastes Agriculture

Problems caused by Pollution Putrefaction of organics – generation of methane and hydrogen sulfide gas. Bad odor. BOD and COD. Growth of pathogenic bacteria- Diseases Waters with BOD cannot be used for irrigation since plant roots need molecular oxygen. Breeding of Mosquito larvae Contamination of ground water.

Problems ….. Proliferation of blue green algae. Drop in fish growth. Pollution in Oceans helps growth of dinoflagellates (red tides) and jelly fish not finfish. Water cannot be used for drinking or other purposes without treatment

CLEANING POLLUTED WATERS Organics having BOD &COD POLLUTION ANAEROBIC BACTERIA Bad Smell Incomplete Treatment Pollutes Ground Water AEROBIC BACTERIA Complete Treatment No bad Smell Requires Oxygen

Current methods of treatment No treatment : Anaerobic bacteria generate Hydrogen sulfide gas and methane- Stink-- Nitrates , nitrites and organics in ground water. Treatment: Sewage treatment plants Aerators – Oxygen --Activated sludge process –Aerobic bacteria – Sludge removal Investment—Power--Running cost--Capacity restriction--Location and land

1 LITRE NUALGI FOR 4 MILLION LITRES OF WATER Nualgi Technology 11LTR 1 LITRE NUALGI FOR 4 MILLION LITRES OF WATER Photosynthesis Diatom Algae grows in water Zooplankton and fishes clean up the water body. Aerobic bacteria breaks down organics Produces pure oxygen by Photosynthesis under water

Nualgi Technology - Benefits Cleaning polluted lakes, rivers, Oceans Global carbon sequestration Repopulating rivers and Oceans with fish. Mosquito control Boosting low cost and high productive agriculture. Growing nutritious food and health benefits.

Nualgi treatment of sewage polluted lake

Sahibabad Drain, Ghaziabad, India Started On 8/10/2015 for 3 months Case Study Of Nualgi Based Treatment at Sahibabad Drain, Ghaziabad, India Started On 8/10/2015 for 3 months

Sahibabad Drain @ Brij Vihar Dosing of Nualgi (Diluted in water)

Observations post Dosing Bubbles of oxygen rising to the surface and creating ripples on the surface

Sludge at the bottom tends to rise to the top and break down due to aerobic bacteria

Pre and Post Treatment Samples in 13 days

Test reports before and after 13 days 7 Total Kjeldahl nitrogen, mg/L 14.84 3.64 -do- 8 Total phosphates, mg/L 59.65 128.64 9 Dissolved oxygen, mg/L 4.8 5.2 IS-3025 (P-38) 10 COD, mg/L 2000 240 APHA Edt 88% decrease in CoD levels in 10 days of treatment. 11 BOD, mg/L 1480 60 95% + decrease in BoD levels in 10 days 12 Nitrate, mg/L 7.36 4.28 13 Silica, mg/L 60.34 784.8 14 Surfactants, mg/L 0.036 15 Total dissolved solids, mg/L 2222 1806 IS-3025 (P-16) 16 Free chlorine residual, mg/L ND 0.6

Test reports before and after 13 days Microbiological Analysis: 1 Total coliform, MPN/100 ml 3,00,000 300 IS-1622 Levels decreased by 99.9% 2 Faecal coliform, MPN/100ml 22000 240 Levels decreased by 98% + Phytoplankton’s: 3 Blue, per ml 8700 6800 Reduced by 20% approx Green per ml 45000 55000 -do- Di-atomic, per ml 15000 17500 Increased by 16.6%

Case study – Al Reem Lake Dubai Al Reem Lake – Pre Nualgi

Al Reem lake Dubai after treatment with Nualgi Al Reem Lake – Post Nualgi

Al Reem Lake – Post Nualgi

Other typical cases Ooty lake treatment is done with Nualgi Lakes Exporting to USA where Lake savers and Clean flo are using this technology to treat lakes Treatment of domestic effluents of 6 lakh litres / day in Brigade Gateway Bangalore using Nualgi technology in existing ETP in the cellar. Blowers have been switched off and output tested meets pollution control norms. LED lamps used for photosynthesis.

Questions?

ADDITIONAL INFORMATION Please view “The diatom Story” 2 parts in Youtube for more information. Two proposals submitted by us to contests organised by the Climate CoLab of Massachusetts Institute of Technology, Cambridge, USA have won first prize.

T. Sampath Kumar, CEO, Nualgi Nanobiotech, Presentation by T. Sampath Kumar, CEO, Nualgi Nanobiotech, 651, 11th main, 5th block, Jayanagar, Bengaluru-560041, India. Tel: (080) 26591524, M 9449767543 Email: sampath651@gmail.com