Robert Camp Mechanical Engineering Extracellular Matrix Engineering Research Laboratory (EMERL) Advisor: Jeff Ruberti.

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Presentation transcript:

Robert Camp Mechanical Engineering Extracellular Matrix Engineering Research Laboratory (EMERL) Advisor: Jeff Ruberti

Agenda Collagen – The protein of interest Review of Research Literature Review Pipette Manufacture Chemistry Current Experiment Setup Question/Comments

What is Collagen? Principal load bearing and most abundant protein in vertebrate animals Defined as (GLY-X-Y) n 3 left-handed helical alpha chains form one right-handed helix collagen monomer Picture Source: Wikipedia.org

Collagen Type I 27 different types of collagen found in nature Collagen Type I is the basis of research One of the fibril forming collagens Type I, II, III, V, XI Monomer Size 300 nanometers long 1.5 nanometers in diameter Picture Source: Wikipedia.org

Review of Research Reading key published works concerning my research Single molecule manipulation Bustamante - Effects of mechanical force on DNA/RNA polymerase activity Magnetic Tweezers Prentiss - Studying the forces in ligand-receptor Complexes (basis of new experiments) Marko - Single DNA strand manipulation (basis of ultimate experiment) Collagen Sun – Mechanical strength of collagen

Bead Catching Apparatus Manufacturing bead catching pipettes This is extremely difficult since the tips are so small Force Measuring Pipette Loading Pipette Bead Catching Pipette Permanent Magnet Source: Yan, et al 2004

Chemistry Shifting the focus of my current research to the chemical bonding of collagen Working with antibodies provided by the NICDR Currently setting up new experiment to test collagen This will be a springboard to my single collagen manipulation experiments It will also provide much statistical data to help support my single collagen experiment

Current Experiment YYY CollagenBind Wash Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Not to Scale 1 Micron Paramagnetic Beads

Apply Force ( pN) Add MMP-8 Bead and Collagen are to scale

“The most exciting phrase to hear in science, the one that heralds new discoveries, isn’t ‘eureka!’, but rather ‘hmmmm, that’s funny’.” Isaac Asimov