PR_7_ИФА PR_8_Проточная цитометрия. Антитело Варианты ИФА.

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Presentation transcript:

PR_7_ИФА PR_8_Проточная цитометрия

Антитело

Варианты ИФА

Схема «Сэндвич» метода ИФА 1. To coat the ELISA platewith diluted capture antibody and incubate overnight at 4°C. 2. Wash the plate wells with ddH 2 O, wash with PBS-Triton twice. 3. Block non-specific binding using 1% BSA/PBS and incubate for minutes at RT. 4. Wash plate. Add standards and 100ul of diluted samples to appropriate wells. 5. Incubate for 1 hour at RT. Wash. 6. Add 100ul appropriate dilution of the secondary antibody conjugated with Alkaline Phosphatase (AP) or Horseradish Peroxidase (HRP) and incubate for 1 hour. Wash. 7. Add 100ul of substrate to well and incubate at RT for 1 hour. (Add stopping solution) 8. Read plates on an ELISA microplate reader.ELISA platestopping solutionmicroplate reader

Проточная цитометрия Flow cytometry

Fluorescence Activated Cell Sorting (FACS)

Холостая проба