SCM 330 Ocean Discovery through Technology Area F GE

Sensors - Biological Traditional Approach Nets Bottles Sensors Fluorometers Optical Scattering (particle size) Flow Cytometers Acoustics VPR Light Sheets Molecular Tagging Theory Application Sensor

Phido-Ø, or also known as Free-Fall, is a two-dimensional imaging fluorometer. It falls through the water column taking high-resolution images of the physical and planktonic microstructures that exists in our oceans. It's an autonomous, untethered instrument composed of a high sensitivity, low noise, thermo-electric cooled CCD camera (LUMIS) and a green (532 nm) laser. The laser light, spread as a thin sheet, is used to induce the chlorophyll from the microstructures, which lights as fluorescence. The camera is equipped with special filters to image this fluorescence. The imaging takes place 75 cm below the instrument and therefore in a completely undisturbed medium. LASER LIGHT SHEETS

The system is broken down into four major components; camera, laser, sensors, and frame.

3d-PIV is a combined multi-spectral imager and particle imaging velocimeter (PIV) profiler. It uses the existing Phido-Ø vehicle frame but uses two cameras taking images simultaneously allowing for the PIV imaging component. These images are used to calculate maps of velocity, vorticity, rate of strain and dissipation rate of turbulent kinetic energy.

Our understanding of the significance of microbes in the ocean has improved dramatically over the last decade. However there is still great need for instruments capable of providing insight into the behavior of microbes in their natural setting. The Thin Laser light Sheet Microscope (TLSM) is a possible solution to this problem.

In a step towards realizing that goal, Chris Scholin has developed the Environmental Sample Processor (ESP), a analysis tool that involves scientists, mechanical, electrical and software engineers. The ESP is an electromechanical/fluidic instrument system designed to collect discrete water samples from the ocean subsurface, concentrate microorganisms (particulates), and automate application of molecular probes to identify microorganisms and their gene products. In addition, the ESP archives discrete samples for nucleic acid analysis, microscopy and other types of analytical procedures after the instrument is recovered. To date, the ESP has focused on a number of marine planktonic organisms ranging from heterotrophic and photosynthetic bacteria, archaea and eukarya to small invertebrates

Core Sample Acquisition & Analysis System Suitable for collecting small to moderate sized samples (ml’s to 2L) Automates sample archival, development of DNA probe arrays and competitive ELISAs in situ.

Whole Cell Formats

Cell-free Formats