C a p a 1 Universidade de São Paulo Faculdade de Filosofia Ciencias e Letras de Ribeirão Preto Departamento de Química Voltammetric detection of LSD using a chemically modified carbon paste electrode with Schiff base complex Marcelo Firmino de Oliveira

Farmaceutical identifiers Drug analysis by american CSIs and Brazilian Federal Police Class A Class B Class C FTIR   CE Spot tests MS GC fluorescence H-RMN TLC Immunological assay Raman HPLC Melting point Optical microscopy UV-Vis Farmaceutical identifiers A+A A+B A+C B+B+B B+B+C PS: no electrochemical methods cited

Cell, electrodes and expected results in voltammetry

Example of electrochemical analysis in forensic chemistry: Cocaine (SWV: LOD of 1nmol/L) V. Pavlova et al. / Analytica Chimica Acta 512 (2004) 49–56

LSD 1938 – Dr. Albert Hofmann Lysergic acid + diethylamide d-LSD d-iso-LSD l-iso-LSD l-LSD 1938 – Dr. Albert Hofmann Lysergic acid + diethylamide Actuation: CNS hallucinations; euphoria; panic; confusion; psychotic episodes.

Chemical modifier Schiff Bases: 1864 – Hugo Schiff Amine + Aldehyde/Ketone applicability : antimicrobial Fungicide Herbicide Anticancer Complexation with metallic centers: UO22+

OBJECTIVES Apply the complex [UO2(Ac-ophen)]·H2O as carbon paste electrode modifier; Voltammetric detection and quantification of LSD.

Experimental 40% 40% 20% CV SWV DPV KCl 0.1M

Results Standard addition – SWV Frequency: 10 Hz Amplitude: 800 mV

Standard addition - DPV Amplitude: 800mV pulse time: 40ms

Voltammetric technique SD (μA) m (μA L μmol-1) LOD (μmol L-1) LOQ SWV 0.992 1.79x10-2 1.75x10-1 0.31 1.02 DPV 0.984 2.63x10-2 1.52x10-1 0.52 1.73

CONCLUSIONS A new transductor for LSD analysis; Detection and quantification of LSD μmol L-1 range; Good stability for the proposed CM-CPE in water. Thank you