 Biochemistry Unit 2. Atoms  Atom – the basic unit of matter.  Contains subatomic particles:  Protons (+ charge)  Neutrons (no charge or neutral)

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Presentation transcript:

 Biochemistry Unit 2

Atoms  Atom – the basic unit of matter.  Contains subatomic particles:  Protons (+ charge)  Neutrons (no charge or neutral)  Electrons (- charge)  Protons and neutrons have about the same mass. Electrons are much smaller.  Atoms have equal number of protons and electrons.  Because these particles are opposite charges, the whole atom is neutral.

Elements and Isotopes  Element – a pure substance that conssits of only one type of atom. Elements are represented by a 1 or 2 letter symbol. (Example: C = carbon)  The number of protons in an element is that element’s atomic number.  The atomic number of Carbon is 6, therefore Carbon has 6 protons and consequently 6 neutrons.  Mass Number – the sum of the number of protons plus the number of neutrons. (Protons + Neutrons = Mass Number)

Isotopes  Isotopes – atoms of the same element that have different number of neutrons.  Isotopes are identified by their mass number (Examples: carbon- 12, carbon-13, carbon-14)  The weighted average of the masses of an element’s isotopes is called its atomic mass.  Isotopes have the same number of protons and electrons, so all isotopes are neutral. However, since isotopes have different numbers of neutrons, their masses are different.

Radioactive Isotopes  Some isotopes are radioactive. This means that their nuclei are unstable and break down at a continuous rate over time.

Ions  Ion – an atom that has gained or lost an electron, giving it a positive or negative charge.  Cation – a positively charged ion  Anion - a negatively charged ion

Chemical Compounds  Compound - formed by the chemical combination of two or more elements in definite proportions.  Compounds are typically written as a chemical formula (Example: NaCl, CO 2, H 2 O, etc.). This give you the ratio of elements in the compound.  The physical and chemical properties of a compound are usually very different from the elements from which it was formed.  Ionic Compounds – form between a metal and non-metal  Molecular Compounds – form between two non-metals

Chemical Compounds  Chemical compounds are held together by bonds, which are formed by the electrons of each element.  The electrons that are available to form bonds are called valence electrons, and are in the outermost energy level.

Chemical Bonds  Ionic Bonds – formed when one or more electrons are transferred from one atom to another.  Form between a metal and nonmetal.  Creates a cation and anion.

Chemical Bonds  Covalent Bonds – results when electrons are shared between atoms.  The structure that results when atoms are joined together by covalent bonds is called a molecular compound or molecule.  Covalent bonds form between two non-metals.  Because of the large amounts of water in living things, covalent bonds are the only types of bonds found in living things.

Chemical Bonds  Single Covalent Bond – atoms share 2 electrons.  Double Covalent Bond – atoms share 4 electrons.  Triple Covalent Bond – atoms share 6 electrons.

Forces Between Molecules  Intramolecular Forces – between atoms in a molecule.  Example: covalent bonds  Intermolecular Forces – between atoms of two different molecules.  Examples: Hydrogen bonding, Van der Waals forces

Intermolecular Forces  Hydrogen Bonding – hydrogen can form a special types of bonds with a couple of unique elements:  Fluorine  Oxygen  Nitrogen  Hydrogen bonds are represented by dotted lines between atoms of different molecules.

Intermolecular Forces  Van der Waals forces – slight attraction that develops between oppositely charged regions of nearby molecules.  These forces are not as strong as ionic, covalent, or hydrogen bonds although they can hold molecules together.

The Water Molecule  The single most abundant compound in living things.  Water expands as it freezes.  Water is a neutral molecule (equal number of protons and electrons)

The Water Molecule  Water is polar because there is an uneven distribution of electrons between the oxygen and hydrogen atoms.  The hydrogen ends of the molecule have a slightly positive charge and they oxygen end has a slightly negative charge.

The Water Molecule  Because of the opposite charges, polar molecules (such as water) can attract each other.  The attraction between the hydrogen atom on one molecule and the oxygen atom on another molecule is an example of a hydrogen bond.

Properties of Water  Cohesion – an attraction between molecules of the same substance. (Example: water attracted to water)  Adhesion – an attraction between moelcules of different substances. (Example: water attracted to glass, meniscus)

Properties of Water  Water also has a high surface tension which allows it to form a film-like surface.  Water has a high surface tension due to the strong cohesive forces between water molecules.  Capillary Action – the force that allows water to move up against the force of gravity.  Example: water being absorbed up the roots in plants.

Solutions and Suspensions  Heterogeneous Mixtures – composed of two or more elements or compounds that are physically mixed together, but not chemically combined.  Homogeneous Mixtures (Solutions) – all components are evenly distributed throughout the solution  Solute – the substance that is dissolved  Solvent – the substance in which the solute dissolves  Suspensions – mixture of water and non-dissolved materials.

Acids, Bases, and pH  pH Scale – indicates the concentration of hydrogen (H+) ions in solution. This scale ranges from 0-14.

Acids, Bases, and pH  Acids – have a pH value below 7.  Strong acids have a pH between 1-3. (Example: HCl)  Acids have a high concentration of hydrogen (H+) ions, and a low concentration of hydroxide (OH-) ions.  Bases - have a pH value above 7.  Strong bases have a pH between (Example: NH 3 )  Bases have a high concentration of OH- ions, and a low concentration of hydrogen (H+) ions.

Acids, Bases, and pH  Buffers – weak acids or bases that can react with strong acids or bases to prevent sharp, sudden changes in pH.  Neutral – pH = 7  Distilled water has neutral pH.

Carbon Compounds  Organic Chemistry – the study of all compounds that contain carbon.  Macromolecules – made from thousand of smaller molecules.  Polymerization – large compounds called polymers are built by joining smaller compounds (called monomers) together.

Carbon Compounds  There are four groups of macromolecules found in living things:  Carbohydrates – made of monosaccharides  Lipids – made of glycerol and fatty acid  Nucleic Acids – made of nucleotides  Proteins – made of amino acids

Carbon Compounds MacromoleculeMonomerPolymer Carbohydrates Monosaccharide (simple sugars) Polysaccharides LipidsGlycerol, fatty acids Triglycerides, phospholipids, steroids, cholesterol ProteinsAmino acidsPolypeptide Nucleic Acids Nitrogenous base, phosphate group, and sugar (nucleotide) DNA, RNA, and ATP

Carbohydrates  Carbohydrates – compounds made up of carbon, hydrogen, and oxygen atoms, usually in a 1:2:1 ratio.  Living things use carbohydrates as their main source of energy.  Plants and some animals also use carbohydrates for structural purposes. (Examples: cellulose in plant walls)  Monosaccharides – single sugar molecules. (Examples: glucose)  Polysaccharides – large macromolecules formed from monosaccharides. (Example: glycogen, cellulose, chitin)

Lipids  Lipids – made mostly from carbon and hydrogen atoms, which combine to form fatty acids and glycerol.  The common categories of lipids includes fats, oils, waxes, and steroids.  Lipids can be used to store energy.  If each carbon atom in a lipid’s fatty acid chain is joined to another carbon atom by a single bond, the lipid is said to be saturated, because is contains the maximum number of hydrogen atoms. Saturated fats will be solids at room temperature.  If there is at least one double bond between carbon atoms in a fatty acid chain, the lipid is said to be unsaturated. Unsaturated fats will be liquids at room temperature.

Fatty Acid ChainsTriglycerides

Nucleic Acids  Nucleic Acids – macromolecules containing hydrogen, oxygen, carbon, and phosphorous.  Nucleic acids are polymers assembled from individual monomers known as nucleotides.  Nucleotides consist of 3 parts:  Nucleic acids store and transmit hereditary or genetic information as RNA and DNA, and help control the production of proteins. Nucleotide

Proteins  Proteins – macromolecules that contain nitrogen, carbon, hydrogen, and oxygen.  Proteins are made of monomers called amino acids which are held together by peptide bonds.  Amino acids are compounds with an amino group (-NH 2 ) on one end and a carboxyl group (-COOH) on the other end.

Proteins  The instructions for arranging amino acids into many different proteins are stored in DNA.  Each proteins has a specific role  Some proteins control the rate of reactions and regulate cell processes.  Some are used to form bones and muscles.  Others transport substances into or out of the cells.  Some help fight disease.  Proteins have 4 levels of organizations: primary, secondary, tertiary, and quaternary.

Protein Structure

Chemical Reactions  Chemical Reaction – a process that changes, or transforms one set of chemical into another.  Reactants – the elements or compounds that enter into a chemical reaction (on the left of the equation).  Products – the elements or compounds produced by a chemical reaction (on the right of the equation).  Chemical reactions always involve changes in the chemical bonds that join atoms in compounds. 2 H 2 + O 2  2 H 2 O

Energy of Reactions  Exothermic (Catabolic) Reactions – break bonds and release energy  Endothermic (Anabolic) Reactions – forms bonds and requires energy  Exothermic reactions that release energy often occur spontaneously. Endothermic reactions that absorb energy will not occur without a source of energy to initiate the reaction.

Activation Energy  Activation Energy – the energy that is needed to get a reaction started.

Enzymes  Catalyst – a substance that speeds up a chemical reaction.  Catalysts work by lowering a reaction’s activation energy.  Enzymes – proteins that act as biological catalysts.  Enzymes speed up chemical reactions that take place in cells.

Enzyme Action  Enzymes area very specific, generally catalyzing only 1 chemical reaction.  Substrates – the reactants of enzyme-catalyzed reactions (what the enzyme acts on; used instead of reactants).  Active Site – place on the enzyme where the substrate binds. (Example: see slide 1)

Enzyme-Substrate Complex

Regulation of Enzyme Activity  Enzymes work best at certain pH values.  Many enzymes are affected by changes in temperature.  Enzymes play essential roles in regulating chemical (metabolic) pathways, making materials that cells need, releasing energy, and transferring information.