Lecture 14 IR & 1H NMR Practice Problem (Lec 13)

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Presentation transcript:

Lecture 14 IR & 1H NMR Practice Problem (Lec 13) Column Chromatography of Plant Pigments Get Lecture Problem 5, due next class This Week In Lab: Ch 7 PreLab Due Quiz 4 Ch 5 Final Report Due Next Week in Lab: Ch 9 PreLab Due Quiz 5 Ch 6 Final Report Due

Column Chromatography of Plant Pigments Chapter 9 Column Chromatography of Plant Pigments A two-day experiment Day One - Extraction & Chromatography of Plant Pigments Day Two - UV/Vis Analysis of Plant Pigments

Extraction of Plant Pigments Obtain spinach leaves Grind up leaves with acetone, petroleum ether and CaCO3 Transfer to a separatory funnel Extract (similar to Ch 5!) Organic layer contains plant pigments - to be separated via column chromatography

Column Chromatography of Plant Pigments Pack column Load sample (plant pigment solution/organic layer) to column Run column & collect fractions in tared flasks **NEVER let the column to dry** Always have fresh mobile phase in the column TLC the fractions See video on column prep in class.

Getting the column ready Column Chromatography Of Plant Pigments: Step 1 Getting the column ready http://www.uni-regensburg.de/Fakultaeten/nat_Fak_IV/Organische_Chemie/Didaktik/Keusch/D-CC-e.htm

What the column will look like - the colored bands are the plant pigments! Running the column & collecting fractions http://www.uni-regensburg.de/Fakultaeten/nat_Fak_IV/Organische_Chemie/Didaktik/Keusch/D-CC-e.htm

Evaluating the fractions Finishing up & Evaluating the fractions The different fractions That contain the Different pigments Evaluate by TLC & By UV/Vis http://www.uni-regensburg.de/Fakultaeten/nat_Fak_IV/Organische_Chemie/Didaktik/Keusch/D-CC-e.htm

Carotenoids Hydrocarbons Orange/red/yellow pigments in fruits and veggies Conjugated p systems Involved in photosynthesis

Chlorophylls Porphyrins Blue/green pigments in plant leaves Also involved in photosynthesis Produced by plants in response to sunlight

Flavonoids Aromatic compounds Red, violet, blue, or yellow pigments in plants, fruit, flowers In fruit and flowers, act as an attractant for insects to distribute pollen and seeds In leaves, act as sunscreens