Bio 328 Immunology Immunoglobulins: structure and Function

+ - Albumin  globulin  globulin  globulin Origin InflammationNormal Myeloma

Arne Tiselius and Elvin Kabat 1939.

2014) 1972 Noble Prize in Medicine.

Porter et al. Nisonoff et al. Edelman et al.

Goat anti-Human Fab binds to both L- and H-chain; goat antiHuman Fc binds only to H-chain.

Angiotensin ll

Van der Waals surface contact between angiotensin ll and Fab fragment.

Flu virus antigen and antibody

Fab and Influenza virus antigen

Anti-HIV protease Fab + peptide

Fab different anti-hen egg lysozyme Fab different small epitopes

UnboundAg-bound Induced-fit

UnboundAg-bound Anti-hemagglutinin Fab and peptide.

Antibody –Mediated Effector Functions 1.Ab-medicated clearance of pathogens a)Neutralization b)Opsonization c)Complement activation d)ADCC 2.Ab isotypes or classes mediate different functions 3.Fc Receptors

How do Antibodies kill Cancer Cells? 1.Binding to cell surface receptors and preventing their activation. 2.Interference with vascularization. 3.Induction of apoptosis. 4.Induction of ADCC. 5.Induction of Complement fixation. 6.Delivery of toxins.

IgG, IgA, IgDIgM, IgE C H 1/C H 1 Hinge region C H 2/C H 2 C H 3/C H 3 C H 4/C H 4

1921 K. Prausnitz and H. Kustner 1966 K and Y Ishizaka

Immunoreceptor Tyrosine Activation Motifs

B-cell Linker Protein Bruton’s tyrosine kinase

X-linked agammaglobulinemia

The End