Role of V3 in HIV Entry and Neutralization Chloe Jones, Isabel Gonzaga, and Nicole Anguiano BIOL398: Bioinformatics Laboratory Loyola Marymount University.

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Role of V3 in HIV Entry and Neutralization Chloe Jones, Isabel Gonzaga, and Nicole Anguiano BIOL398: Bioinformatics Laboratory Loyola Marymount University 10/15/14

Outline - V3 plays a critical role in coreceptor binding in gp120 protein to CD4 T cells -Figures: - - Superimposing antibodies on V3 structure with core blocks coreceptor binding -Accessibility of antibodies to V3 aids in immunodominance -Studying the structure of V3 can aid in the understanding of the neutralization and progression of the HIV virus

HIV Envelope Structure Allows for Viral Entry to T-Cells ●HIV envelope has trimeric spike ○ Contains 3 gp120 exterior 3 gp41 transmembrane glycoproteins ●Host CD4 T-Cells bind to gp120 and causes conformational change

V3 Region Critical to Coreceptor Binding Site

Superimposing antibodies on V3 structure with core blocks coreceptor binding -Monoclonal antibodies 50.1, 58.2, 59.1, 83.1, and D bind to conserved V3 tip blocking coreceptor binding.

Accessibility of antibodies to V3 aids in immunodominance -Two different views of the accessibility -Antibodies bind to either the core or V3 -V3 engulfed in neutralizing antibodies, proposing a role in immunization.

Significance of the Article -V3 plays a major role in the establishment of the HIV virus within the body -essential in coreceptor binding -Coreceptor interaction and altering quaternary interactions of V3 can aid in HIV avoiding immune system and HIV entry into the cells -From studying the V3 structure, the HIV virus it can be further examined and analyzed towards progression and neutralization

Citation Huang, C. C., Tang, M., Zhang, M. Y., Majeed, S., Montabana, E., Stanfield, R. L., Dimitrov, D. S., Korber, B., Sodroski, J., Wilson, I. A., Wyatt, R., & Kwong, P. D. (2005). Structure of a V3- containing HIV-1 gp120 core. Science, 310(5750), DOI: /science