Overview of Mass Spectrometry

Slides:

Advertisements

Similar presentations

Genomes and Proteomes genome: complete set of genetic information in organism gene sequence contains recipe for making proteins (genotype) proteome: complete.

Advertisements

Protein Quantitation II: Multiple Reaction Monitoring

Improvements in Mass Spectrometry for Life Science Research – Does Agilent Have the Answer? Ashley Sage PhD.

The Proteomics Core at Wayne State University

UC Mass Spectrometry Facility & Protein Characterization for Proteomics Core Proteomics Capabilities: Examples of Protein ID and Analysis of Modified Proteins.

In-depth Analysis of Protein Amino Acid Sequence and PTMs with High-resolution Mass Spectrometry Lian Yang 2 ; Baozhen Shan 1 ; Bin Ma 2 1 Bioinformatics.

By Jonathan Haltiwanger and Teresa Leslie.  Analytical technique to determine the elemental composition of a sample  Vaporization  ionization  electric.

Mass Spectrometry in the Biosciences: Introduction to Mass Spectrometry and Its Uses in a Company Like Decode. Sigurður V. Smárason, Ph.D. New Technologies.

Proteomics The proteome is larger than the genome due to alternative splicing and protein modification. As we have said before we need to know All protein-protein.

PROTEIN IDENTIFICATION BY MASS SPECTROMETRY. OBJECTIVES To become familiar with matrix assisted laser desorption ionization-time of flight mass spectrometry.

Protein Identification and Peptide Sequencing by Liquid Chromatography – Mass Spectrometry Detlef Schumann, PhD Director, Proteomics Laboratory Department.

Lawrence Hunter, Ph.D. Director, Computational Bioscience Program University of Colorado School of Medicine

Proteomics Informatics – Protein identification II: search engines and protein sequence databases (Week 5)

Announcements: Proposal resubmissions are due 4/23. It is recommended that students set up a meeting to discuss modifications for the final step of the.

Proteomics Informatics Workshop Part I: Protein Identification

Vermont Genetics Network Outreach Proteomics Module

Previous Lecture: Regression and Correlation

HOW MASS SPECTROMETRY CAN IMPROVE YOUR RESEARCH

Mass Spectrometry. What are mass spectrometers? They are analytical tools used to measure the molecular weight of a sample. Accuracy – 0.01 % of the total.

Proteomics Informatics (BMSC-GA 4437) Course Director David Fenyö Contact information

My contact details and information about submitting samples for MS

Proteomics Josh Leung Biology 1220 April 13 th, 2010.

Proteomics Informatics (BMSC-GA 4437) Course Director David Fenyö Contact information

Proteomics Informatics Workshop Part II: Protein Characterization David Fenyö February 18, 2011 Top-down/bottom-up proteomics Post-translational modifications.

Tryptic digestion Proteomics Workflow for Gel-based and LC-coupled Mass Spectrometry Protein or peptide pre-fractionation is a prerequisite for the reduction.

Mass Spectrometry Mass spectrometry (MS) is not true “spectroscopy” because it does not involve the absorption of electromagnetic radiation to form an.

Comparison of chicken light and dark meat using LC MALDI-TOF mass spectrometry as a model system for biomarker discovery WP 651 Jie Du; Stephen J. Hattan.

Chapter 9 Mass Spectrometry (MS) -Microbial Functional Genomics 조광평 CBBL.

2007 GeneSpring MS GeneSpring for Metabolite BioMarker Analysis using Mass Spectrometry data Agilent Q-TOF VIP Visit Jan 16-17, 2007 Santa Clara, CA Thon.

PROTEIN STRUCTURE NAME: ANUSHA. INTRODUCTION Frederick Sanger was awarded his first Nobel Prize for determining the amino acid sequence of insulin, the.

Pharmaceutical analysis Bioavailability studies Drug metabolism studies, pharmacokinetics Characterization of potential drugs Drug degradation product.

Mass Spectrometry I Basic Data Processing. Mass spectrometry A mass spectrometer measures molecular masses. The mass unit is called dalton, which is 1/12.

Common parameters At the beginning one need to set up the parameters.

1 Chemical Analysis by Mass Spectrometry. 2 All chemical substances are combinations of atoms. Atoms of different elements have different masses (H =

Temple University MASS SPECTROMETRY INTRODUCTION Ilyana Mushaeva and Amber Moscato Department of Electrical and Computer Engineering Temple University.

Lecture 9. Functional Genomics at the Protein Level: Proteomics.

Genome of the week - Enterococcus faecalis E. faecalis - urinary tract infections, bacteremia, endocarditis. Organism sequenced is vancomycin resistant.

In-Gel Digestion Why In-Gel Digest?

Proteomics What is it? How is it done? Are there different kinds? Why would you want to do it (what can it tell you)?

CSE182 CSE182-L11 Protein sequencing and Mass Spectrometry.

Separates charged atoms or molecules according to their mass-to-charge ratio Mass Spectrometry Frequently.

Proteomics Informatics (BMSC-GA 4437) Instructor David Fenyö Contact information

Salamanca, March 16th 2010 Participants: Laboratori de Proteomica-HUVH Servicio de Proteómica-CNB-CSIC Participants: Laboratori de Proteomica-HUVH Servicio.

Click to add Text Sample Preparation for Mass Spectrometry Sermin Tetik, PhD Marmara University July 2015, New Orleans.

Proteomics Informatics (BMSC-GA 4437) Course Directors David Fenyö Kelly Ruggles Beatrix Ueberheide Contact information

2014 생화학 실험 (1) 6주차 실험조교 : 류 지 연 Yonsei Proteome Research Center 산학협동관 421호

Constructing high resolution consensus spectra for a peptide library

What is proteomics? Richard Mbasu and Ben Richards.

Introduction to Liquid Phase Mass Spectrometry

Computational Challenges in Metabolomics (Part 1)

RANIA MOHAMED EL-SHARKAWY Lecturer of clinical chemistry Medical Research Institute, Alexandria University MEDICAL RESEARCH INSTITUTE– ALEXANDRIA UNIVERSITY.

Yonsei Proteome Research Center Peptide Mass Finger-Printing Part II. MALDI-TOF 2013 생화학 실험 (1) 6 주차 자료 임종선 조교 내선 6625.

An introduction to Mass spectrometry. What is mass spectrometry? Analytical tool measuring molecular weight (MW) of sample Only picomolar concentrations.

Post translational modification n- acetylation Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying unknown protein. Proteins to.

Shotgun protein identification Creative Proteomics offers iTRAQ protein quantification service suited for unbiased untargeted biomarker discovery. Relative.

Richard Mbasu and Ben Richards

Mass Spectrometry makes it possible to measure protein/peptide masses (actually mass/charge ratio) with great accuracy Major uses Protein and peptide identification.

The Syllabus. The Syllabus Safety First !!! Students will not be allowed into the lab without proper attire. Proper attire is designed for your protection.

Mass Spectrometry Vs. Immunoassay

Mass Spectrometry Market Research Report 2022

Thomas BOTZANOWSKI & Blandine CHAZARIN

Proteomic Approaches to Cancer Biomarkers

Interpretation of Mass Spectra I

Figure 1 Schematic representation of a typical MALDI-MSI workflow

A perspective on proteomics in cell biology

Mass Spectrometry THE MAIN USE OF MS IN ORG CHEM IS:

Shotgun Proteomics in Neuroscience

Interpretation of Mass Spectra

Presentation transcript:

Overview of Mass Spectrometry Sermin Tetik, PhD Marmara University July 2015, New Orleans

An overwiev What is the mass spectrometer? What can mass spectrometers identify? What are the different types of mass spectrometers? Peptide fragmentation Databese searching Practical applications of mass spectrometry

What is a mass spectrometer A. It measure mass B. It can give information about chemical structure

1. Sample preparation 2. Ion source Generates ions 3.Mass analyzer Separates ions 4. Detector Mass spectrum 5. Analysıs

John B. Fenn Koichi Tanaka

The mass-to-charge ratio is often referred to as m/z and is typically unitless m: the mass number (atomic mass/U) z: the charge number (Q/e)

-Drug discovery Determine structures of drugs and metabolites Screen for metabolites in biological systems -Clinical testing Perform forensic analyses such as confirmation of drug abuse Detect disease biomarkers (e.g. newborns screened for metabolic diseases)

-Geology Carbon Dating -Environment -Residual gases -Trace contaminants and toxins -Test water quality or food contamination

B. Proteomics Identification of biological material (proteins, nucleic acids, lipids) Determine protein structure , function, folding and interactions Detect specific post-translational modifications throughout complex biological mixtures Quantitate (relative or absolute) proteins in a given sample Monitor enzyme reactions, chemical modifications and protein digestion

A. Top-down proteomics: Identification of intact proteins

B. Bottom-up proteomics: Identification of intact proteins

B. Bottom-up proteomics: Identification of intact proteins

Top-Down VS Bottom-up proteomics:

Top-Down (+) Access the complete protein sequence Abilty to locate post translational modifications (PTMs) Time consuming protein digest is eliminated

Top-Down (-) Complex spectra obtained limits approach to single protein or simple mixtures Does not work well with proteins > 50 kDa

Bottom-up (+) Most widely used approach for proteın ID Reverse phase HPLC provides high-resolution separation of peptide digests Can analyze very complex mixture

Bottom-up (-) Only a fraction of the total peptide population of a given protein is identified (loss PTMs identified) Loss of information about low abundant peptides is mass spectra dominated by high abundance species

What are the different types of mass spectrophotometers? A. MALDI(Matrix assisted laser desorption/ionization)-TOF (Time of flight) B. LC/MS-MS

A. MALDI(Matrix assisted laser desorption/ionization)-TOF (Time of flight)

LC/MS-MS

Peptide Fragmentation by MS/MS

Peptide Fragmentation by MS/MS

Database Searching by MS/MS

Database Searching by MS/MS

Database Searching by MS/MS

Database Searching by MS/MS

Practical applications for mass spectrometry

Identify purified complexes to generate protein-protein interaction

Identify purified complexes to generate protein-protein interaction

Identify purified complexes to generate protein- protein interaction

Thank You