VACCINOLOGY (BIO-301) Credit Hrs 3(2-1) Dr. Aneela javed.

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Presentation transcript:

VACCINOLOGY (BIO-301) Credit Hrs 3(2-1) Dr. Aneela javed

VACCINE PRODUCTION

CO 2 Incubators Water Jacketed CO 2 incubator 3 Gas/CO 2 Incubator with RH Control – Precise control of Oxygen levels combined with CO 2, N 2 and RH ensure accurate conditions for applications such as, hypoxic cell studies and cancer research.

CELL CULTURE

CRYO VIALS

Revival of frozen cells Growth of the cells in adequate media with 1.serum (FCS/FBS) 2.antibiotics Complete media, Importance of aliquots

serum

ADHERENT CELL LINES SUSPENSION CELL LINES

Environment: – Temperature: 37°C (34 °C, 41 °C) – High humidity – 5% CO 2 Split: Trypsin-EDTA Count of Cells (Thrypan Blue)

Laminar Flow HOOD HEPA filter rated at 99.99% efficient for 0.3 micron particulates. The HEPA filtered air is then directed vertically across the work surface.