TGF-β1 Release from Biodegradable Polymer Microparticles: Its Effects on Marrow Stromal Osteoblast Function by Lichun Lu, Michael J. Yaszemski, and Antonios.

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Presentation transcript:

TGF-β1 Release from Biodegradable Polymer Microparticles: Its Effects on Marrow Stromal Osteoblast Function by Lichun Lu, Michael J. Yaszemski, and Antonios G. Mikos J Bone Joint Surg Am Volume 83(1 suppl 2):S82-S92 April 1, 2001 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 1-A and 1-B Cumulative release kinetics of transforming growth factor-&betabeta;1 (TGF- &betabeta;1) from poly(DL-lactic-co-glycolic acid) PLGA/poly(ethylene glycol) (PEG) microparticles as a function of incubation time: microparticles with varied PEG... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 1-A and 1-B Cumulative release kinetics of transforming growth factor-&betabeta;1 (TGF- &betabeta;1) from poly(DL-lactic-co-glycolic acid) PLGA/poly(ethylene glycol) (PEG) microparticles as a function of incubation time: microparticles with varied PEG... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Cumulative release kinetics of transforming growth factor-&betabeta;1 (TGF-&betabeta;1) from poly(DL-lactic-co-glycolic acid) (PLGA)/ poly(ethylene glycol) (PEG) microparticles in pH 7.4 phosphate buffered saline (PBS) for varied initial TGF-&betabeta;1 loa... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 3-A and 3-B Decrease of weight average molecular weight (Mw) of poly(DL-lactic-co- glycolic acid) (PLGA) in PLGA/poly(ethylene glycol) (PEG) microparticles as a function of incubation time: microparticles with varied PEG contents in pH 7.4 phosphate bu... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 3-A and 3-B Decrease of weight average molecular weight (Mw) of poly(DL-lactic-co- glycolic acid) (PLGA) in PLGA/poly(ethylene glycol) (PEG) microparticles as a function of incubation time: microparticles with varied PEG contents in pH 7.4 phosphate bu... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 4-A, 4-B, and 4-C Scanning electron micrographs of poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degradation (Fig. 4-A), after 28 days of incubation in pH 7.4 phosphate buffered saline (PBS) (Fi... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 4-A, 4-B, and 4-C Scanning electron micrographs of poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degradation (Fig. 4-A), after 28 days of incubation in pH 7.4 phosphate buffered saline (PBS) (Fi... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 4-A, 4-B, and 4-C Scanning electron micrographs of poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degradation (Fig. 4-A), after 28 days of incubation in pH 7.4 phosphate buffered saline (PBS) (Fi... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 5-A, 5-B, and 5-C Fluorescence micrographs showing the distribution of fluorescein isothiocynate-labeled bovine serum albumin (FITC-BSA) within poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degr... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 5-A, 5-B, and 5-C Fluorescence micrographs showing the distribution of fluorescein isothiocynate-labeled bovine serum albumin (FITC-BSA) within poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degr... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 5-A, 5-B, and 5-C Fluorescence micrographs showing the distribution of fluorescein isothiocynate-labeled bovine serum albumin (FITC-BSA) within poly(DL-lactic-co-glycolic acid) (PLGA)/poly(ethylene glycol) (PEG) microparticles with 5% PEG: before degr... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 6-A and 6-B Alkaline phosphatase (ALP) activity of marrow stromal cells after 3 days of in vitro culture on poly(propylene fumarate) (PPF) and tissue-culture polystyrene (TCPS) substrates in the presence of different transforming growth factor-&betabe... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 6-A and 6-B Alkaline phosphatase (ALP) activity of marrow stromal cells after 3 days of in vitro culture on poly(propylene fumarate) (PPF) and tissue-culture polystyrene (TCPS) substrates in the presence of different transforming growth factor-&betabe... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.

Figs. 7-A, 7-B, 7-C, and 7-D Marrow stromal cells were assayed for 3H-thymidine uptake (Fig. 7- A), total cell number (Fig. 7-B), alkaline phosphatase (ALP) activity (Fig. 7-C), and osteocalcin production (Fig. 7-D) during 21 days of in vitro culture on poly... Lichun Lu et al. J Bone Joint Surg Am 2001;83:S82-S92 ©2001 by The Journal of Bone and Joint Surgery, Inc.