Muhammad Ashfaq Pakistan barcode project: progress and applications.

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Presentation transcript:

Muhammad Ashfaq Pakistan barcode project: progress and applications

Pakistan Agro-based economy but crops are plagued with pests and diseases

2010: Barcoding economically important insect species from Pakistan Biodiversity Institute of OntatioNational Institute for Biotechnology and Genetic Engineering (NIBGE) Funded by: HEC

Project execution Involvement of universities/ research institutesMuseumsFaculty/ scientistsGraduate students

Manual catching Pipettes and sieves Arial nets Light traps Shaking onto sheets Activities and Progress Collecting animal parasites

Specimen preparation and databasing

Specimens Animals: Plants: 1289 Barcodes Animals: Plants: 988 IDRC (62%) 18 months

Pests Beneficials

Pakistan-only BINs/ species I.Animals: 4021 II.Plants: 363 Number of BINs/ species 2793 (70%)Singletons: 1530

I.Families: 199 II.Genera: 698 Taxonomic identifications Unidentified: III.Species: 764

Barcode Applications Insect pests II.Identification and distribution of:I.Barcode reference libraries Disease vectors

Reference libraries Pests:754 speciesBeneficials:241 species

Noctuid species in/around cotton fields of Punjab Previously only S. litura was reported from cotton in Punjab

Genetic diversity: mango mealybug, Drosicha mangiferae

Bemisia tabaci, a complex of cryptic species

(A) Asia II 1 (B) MEAM 1 (C) Asia 1 Bemisia tabaci, distribution analysis

Dengue epidemic in Punjab

Mosquito species in Punjab

Genus: Anopheles Genus: Culex Genus: Aedes Aedes aegypti Aedes albopictus Genus-wise distribution of mosquitoes in Punjab

Outreach Training and involvement of communitySymposiums and seminarsOne-to-one meetingsMedia coverage

Barcoding project at NIBGE Strengths A trained group of workersSpecimen processing facilityDepository for voucher specimens

Concerns No imminent financial support to continue barcoding activities after August 2013

Barcoding team members

Thank you for your attention