By Kacie Capozzoli, Jenny Nguyen, and Chuck Thonsen

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Presentation transcript:

By Kacie Capozzoli, Jenny Nguyen, and Chuck Thonsen How do algae respond to light? Determining the Process of Phototaxis in Chlamydomonas reinhardtii By Kacie Capozzoli, Jenny Nguyen, and Chuck Thonsen

Background/Basis for Experiment Wild type Chlamydomon as reinhardtii move toward white light in the process of phototaxis, but the mutant strain does not. The inability of the mutant to perform phototaxis may be due to the eyespot being too small or weak, and unable to detect certain wavelengths of light.

Specific Questions and Predictions What caused the mutant strains of Chlamydomonas reinhardtii to be immotile and unable to perform phototaxis? Would the eyespot of the mutant strain be able to detect blue light? In presence of both white light and blue light, which type of light will the mutant strain go towards? Biological Prediction: The mutant strain will move towards the blue light, even when competing with the source of white light.

Results TRIAL 1: Phototaxis CC-125 (wildtype) CC-2894 (mutant) control CC-125 (wildtype) CC-2894 (mutant) control blue light (++) white light Physical appearance control- no movement, blue and white light- clumps, but minimal movement towards the light (ring) control - clumps spread out and no movement, blue and white light- moving to light TRIAL 2: Phototaxis CC-125 (wildtype) CC-2894 (mutant) control blue light (++) white light + Physical appearance control- no movement, blue light- clumps, but minimal movement to light, white light- moving to light (very faint) control- clumps spread out and no movement, blue light- moving towards light, white light- clumps, but little movement towards light (ring) COMPETITION TRIAL 2 white light blue light CC-125 (wildtype) CC-2894 (mutant) (++) Physical appearance WT strain- no movement, mutant strain- moving to light COMPETITION TRIAL 1 white light blue light CC-125 (wildtype) (++) CC-2894 (mutant) Physical appearance WT strain- minimal movement towards light, mutant strain- no movement towards light WT strain- moving towards light, mutant strain- no movement