INVESTIGATION THE IN VITRO BINDING EFFICIENCY OF DIFFERENT TOXIN ADSORBENTS ON MYCOTOXINS AND MICROELEMENTS INTRODUCTION One of the most frequently used practical approach against mycotoxins has been the addition of adsorbents to contaminated feed to bind the mycotoxin during the digestive process. Adsorbents can be easily added to animal feeds. However, not all adsorbents are equally effective for each mycotoxin and they have also been shown to impair nutrient, for example mineral and vitamin utilization. OBJECTIVES The main goal of this research was to determine the in vitro binding efficiency of some toxin binders for different mycotoxins and microelements. METHODS Five different commercial adsorbents (Zeolit, Toxy-Nil, Redutox, Mycofix 3.E and Mycosorb) were tested in 4 replicates for ochratoxin A (OTA), zearalenone (ZON), T-2 toxin and deoxynivalenol (DON). Besides testing the toxin binding efficiency, the effect of adsorbents on the iron, copper, manganese and zinc was also determined. The in vitro model employed simulated the in vivo conditions (pH, transit time) of the poultry gastrointestinal tract. The amounts of adsorbents, purified toxins and microelements were determined according to their practical occurrence or recommendations.

RESULTS

CONCLUSIONS The binding efficiency of the investigated adsorbents was toxin dependent. The highest values were found for ZON (57-69%), while the lowest for DON (25- 30%). The highest difference among adsorbents was found in the case of OTA (27-42%). Binding affinity for T-2 toxin ranged between 27 and 37%. Zeolite bound zinc at 11%, the other 3 microelements in a significant higher ratio (34-44%). The other adsorbents showed lower, but still significant affinity for the microelements 12,5-16,5% for cupper, 5-17% for zinc, 6-14% for iron and 6,5- 22,7% for manganese. From the results it can be concluded, that the efficacy of toxin binders used in this experiment is only limited for ZON, DON, OTA and T-2 toxin and they can bind beside mycotoxins also significant amount of micro nutrients. Further in vitro and in vivo studies needed to determine the toxin binder specific mineral and vitamin supplementations of poultry diets, if these types of feed additives are used.