Marbank Marbank -a marine biobank located in Tromsø, Norway Kjersti Lie Gabrielsen

Main objectives Marbank The mission of Marbank is to provide an accessible national repository of frozen marine biological samples, collected and maintained under rigorously controlled conditions The mission of Marbank is to provide an accessible national repository of frozen marine biological samples, collected and maintained under rigorously controlled conditions

Marbank Collection The material to be archived and stored in the Marbank repository will include genetic and biological material from marine microorganisms, plankton, algae, invertebrates and vertebrates The material to be archived and stored in the Marbank repository will include genetic and biological material from marine microorganisms, plankton, algae, invertebrates and vertebrates

Marbank samples Organism/ tissue Water extract Organic extract DNA/ RNA (cDNA) Storage Photos: Klara Stensvåg, NFH

Marbank Storage - LN 2 -tanks and freezers with temperature control/ alarm systems Uniform and high quality storage of samples at low temperatures

Marbank Customers Marbank will offer services for researchers, legislation and industry Marbank will offer services for researchers, legislation and industry Access to samples and data information from Marbank will be regulated by a set of policies Access to samples and data information from Marbank will be regulated by a set of policies R&D institutions and industry will gain access to the material after description of scientific purposes R&D institutions and industry will gain access to the material after description of scientific purposes

Database Marbank Collection Administrative data: -Policies -Loan/transactions Taxonomy/ physical characteristics: -Specie -Name/Common name -Determined by -Weight -Length -Sex/age -Reference literature Field data: - Date -GIS/location -Field prep -Field notes -Hazard -Storage -Voucher Storage: -Tissue/extract -Preservation -Initial weight -Current weight -# individuals -Freezer name etc. -Position in archive (section, rack, box) Digital images: -Photo -Video Literature: - Articles etc. External Server MarbioCustomers Web-site

Marbank strategies for collection of marine samples Initiation of dedicated Marbank surveys/ hiring of research vessels Initiation of dedicated Marbank surveys/ hiring of research vessels Marbank participation on surveys initiated by other institutions Marbank participation on surveys initiated by other institutions Receipt of living or frozen samples collected by researchers from other institutions Receipt of living or frozen samples collected by researchers from other institutions

Marbank initiators/collaborators University of Tromsø University of Tromsø Institute of Marine Research Institute of Marine Research Norwegian Polar Institute Norwegian Polar Institute Norwegian Institute of Fisheries and Aquaculture Research Norwegian Institute of Fisheries and Aquaculture Research

Collaboration Marbank - Marbio CollectionExtractionBioactivityscreeningFractionationPurification Marbank Marbio Database

Marbio: Medium/high throughput screening of novel bioactivities Biomek NX S8 W/ gripper 1 meter ORCA CO 2 incubator Shaker Plate reader Plate washer Plate hotel BC Anti-tumor Anti-tumor Anti-bacterial Anti-bacterial Anti-viral Anti-viral Anti-inflammatory Anti-inflammatory Immunostimulatory Immunostimulatory Enzymes

Summary Marbank will build and maintain a collection of marine samples Marbank will build and maintain a collection of marine samples The frozen tissue samples and extracts will represent the genetic material of a wide range of species The frozen tissue samples and extracts will represent the genetic material of a wide range of species The collection will be an archive of molecular biodiversity and will make unique and important research materials available to the scientific community The collection will be an archive of molecular biodiversity and will make unique and important research materials available to the scientific community