Fundaments of Cell Biology Kristen Lee 9/23/2009.

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Presentation transcript:

Fundaments of Cell Biology Kristen Lee 9/23/2009

Background ProkaryotesEukaryotes  Bacteria  Small & simple  No bound nucleus  Animals, plants, yeast, etc.  Complex metabolism  Nucleus is in membrane-bound compartment 1/09/08. Chasin and Mowshowitz, Department of Biological Sciences, Columbia University Biology 2960 Computer Laboratory, Washington University um um

Cell Membrane  Plasma Membrane Structure  Phospholipid bilayer, proteins, cholesterol Functions:  Selective Barrier  Membrane Transport  Transporters, Channels  Vesicular transport  Cell-cell interactions  Oligosaccharides, Glycoproteins/lipids Royal Society of Chemistry

Cytoskeleton Cytoskeleton TypeDiameterStructure Microfilaments / Actin Filaments 5-9 nmHelical -resist tension, maintaining cell shape, cell-to- X junctions, transduction, cytokinesis Intermediate filaments10 nmRope-like -mechanical stability, anchor organelles, support nuclear envelope Microtubules25 nmHollow cylindrical -rigid, intracellular transport, axoneme of cilia and flagella, component of mitotic spindle, MTOC, centrioles

Protein and Lipid Production  Gene Expression: transcription and translation  Rough ER- studded with ribosomes, folds proteins  Golgi Apparatus- packages/modifies proteins/lipids in vesicles for transport to final destination  Smooth ER- synthesize lipids and steroids, metabolize carbohydrates, regulate Ca concentration

Endomembrane System  Peroxisome- oxidative enzymes metabolize fatty acids, transports proteins  Endosome- sort material that is endocytosed  Lysosome- degrade cytoplasmic unwanteds Energy  Mitochondria- generate most ATP in cells (cell respiration chain, Krebs cycle)

Nucleus National Human Genome Research Institute 46 total chromosomes 2 sex chromosomes (X/Y) 22 pairs of autosomal chromosomes Higher order structures: DNA + histone protein  Chromatin  Chromosome Sister chromatids=2x 1 chromosome Mitotic

Cellular Reproduction  INTERPHASE (genetic material replicates)  MITOSIS  Prophase (chromatin condenses)  Prometaphase (nuclear envelope disassembles, mitotic spindle attaches to chromosomes)  Metaphase (chromosomes align across metaphase plate)  Anaphase (sister chromatids separate)  Telophase (clean-up, reorganization CYTOKINESIS (cleavage furrow)