A&P 120 Enzyme Function Exercise 4 www.celltech.com/resources/vt/enzymes.html.

Slides:



Advertisements
Similar presentations
STEVENSON PBHS AP Biology. Lab #2: Enzyme Catalysis Introduction: This the first of 12 AP Labs designed to illustrate the themes of this class.
Advertisements

Enzymes Chapter 6.3 page165.
Enzymes a special type of protein.
An enzyme is a biological catalyst that speeds up the rate of reactions in biological processes All enzymes are proteins, coded for by genes Enzymes are.
B3. Enzymes Pg
Cells & Enzymes Enzymes Made of protein Present in all living cells Converts substrates into products Biological catalysts Increase the rate of chemical.
Introduction to Enzymes The Chemical Context of Life.
Chapter 5: Enzymes.
Enzymes Enzymes Enzymes: are proteins made of amino acids.Enzymes: are proteins made of amino acids. -Catalyst: they speed up chemical reactions & lower.
IRSC General Biology I Lab
Understanding Enzymes Academic Biology. Enzyme A large protein molecule Specific shape with deep folds on its surface Deep folds form pockets called active.
Enzymes Enzymes Activation Energy (E A ) – the energy required for a chemical reaction to occur.
Enzymes Objective 2.01 Functions of Enzymes How Enzymes Work.
What are the factors affecting Enzyme Activity?. Recap.
Biology 120 Enzyme Function Exercise 4
Enzyme Catalysis Lab. Goals for the Day 1.Describe what an enzyme is and what it does 2.Be able to define activation energy 3.Discuss why enzymes are.
ENZYMES Biological Catalysts 1. ENZYMES ENZYMES are important proteins Many chemical reactions in living cells (and organisms) are regulated by ENZYMES.
What happens to the food that we eat?. IT BREAKS DOWN INTO…. Carbohydrates Proteins Fats NUCLEIC ACIDS!
Homeostasis of Enzymes What is an enzyme? How do enzymes work? What factors can effect enzyme action?
Chemical Reactions Breaking of bonds forming of new ones. New combinations of atoms are produced forming new substances with new properties. Energy needs.
Enzymes Structure and function. Enzymes Over 1000 different reactions can take place in a single cell in any given moment. Each individual reaction is.
ENZYMES. Vocabulary(4 slides are review from last day) Metabolism Anabolic Pathways Catabolic Pathways Free Energy Activation Energy Catalysts.
Enzymes Protein Responsible for most cell functions Functions – to build or break apart – Translate DNA – Breakdown food – Put together macromolecules.
CELL METABOLISM Enzymes Definition Catalysts Proteins that speed up chemical reactions by lowering the energy of activation.
Cells & Enzymes Converts substrates Present in into products
Enzymes.
FACTORS AFFECTING ENZYME ACTIVITY.  There is an optimum temperature at which enzymes function best – generally around 40 degrees  But why the gradual.
Biology Discussion Notes Thursday 10/30/2014. Goals for the Day 1.Be able to determine the reactants and products of a chemical equation. 2.Be able to.
Enzymes in Action Part II
What are enzymes??  Enzymes are proteins.  Enzymes speed up chemical reactions.  Biological Catalyst  pH, temperature, and substrate concentration.
Enzymes A quick guide.
Enzymes AP Biology. General Information Globular proteins Unique 3 dimensional shape Active site: pocket or groove where substrate binds.
Biological catalysts.  Enzymes: proteins inside cells that act as a catalyst.  Proteins = long amino acids chains that are folded into a specific shape.
How enzyme function.
Chemical Reactions Make Life Possible! Chemical Reactions Proceed as Follows: ReactantsProducts Energy must be added to “activate” the reaction and break.
Definitions Characteristics Of Enzymes What lab?Deja.
Enzyme Review Are you ready? Define Catalyst. A molecule that allows stable molecules to react quickly.
Warm-up: August 27, 2012 Make sure your pH lab is completed and turned in, IF YOU HAVE NOT ALREADY.
Enzymes. Chemical Reactions In order for chemical reactions to take place, enzymes must be present to help speed up the reaction. Chemical bonds connect.
Lesson 5 Enzymes. Catalyst: something that increases the rate of reactions Enzymes are biological catalysts Often ends with –ase Most enzymes are proteins.
Enzymes Over 1000 different reactions can take place in a single cell in any given moment. Each individual reaction is catalysed by a specific enzyme.
Enzymes.
Chemistry of Living Things
Catalysts speed up the rate of the reaction.
Enzymes: Biological Catalysts
Topic: Enzymes Aim: Why are enzymes important to living things?
National 5 Proteins & Enzymes Mr G Davidson.
Catalysts speed up the rate of the reaction.
Warm Up Set up your journal for Cornell Notes and title it: Biochemistry- Enzymes Answer #1 and 2.
Enzymes: 1. All enzymes are proteins
Chemistry of Life Enzymes.
Enzymes are a special type of protein!
Enzymes May be used for educational purposes only.
Enzymes How do catalysts speed up chemical reactions?
Biochemistry Enzymes.
Enzymes Unit: Ecology.
Enzymes Enzymes are globular proteins that act as catalysts and help complex reactions occur.  Cytochrome P450 Liver Enzyme.
Enzymes: “Helper” Protein molecules
Living Cells Enzymes Mr G Davidson.
Enzymes a special type of protein.
Topic: Enzymes Aim: Why are enzymes important to living things?
8.5 Enzyme Activity Enzyme Action
Enzymes Kathryn Blair, M.S. BIO 101 Core Concepts
What are enzymes?. What are enzymes? ENZYMES ARE A SPECIAL TYPE OF PROTEIN! Enzymes are Biological catalysts that increase the rate of metabolic reactions.
Chemistry of Living Things
Enzymes Necessary Proteins.
Enzymes Special Proteins.
What DNA sequence codes for this chain of amino acids...?
U01L04: ENZYMES.
o They are mainly proteins o They are biological catalysts that speed up the rate of the biochemical reaction.
Presentation transcript:

A&P 120 Enzyme Function Exercise 4

Terminology Metabolism –Definition: The sum total of all biochemical activity that takes place in a living organism Catabolic Metabolism – break down –AB = A & B Anabolic Metabolism – build up –A + B = AB In vitro – out of the body (or in a tube) –In Vivo – in the body Substrate - The material being acted upon

Introduction Enzyme – a protein that is a organic catalyst –Hint: Most of the time ends in –ase Catalyst – speeds up the rate of a reaction –Catalase – is the name of an enzyme in your body Active Site – a pocket at the outside of the enzyme specific to a substrate (here is where it is taken apart or put together) 2H 2 O 2 2H 2 O +O 2 Catalase (organic) (Hydrogen peroxide) Substrate (Water & Oxygen) Product **Oxygen will bubble in vitro** Bubbles Catalase H2O2H2O2 H2O2H2O2

Temperature Denature: enzyme unravels/unfolds and loses structure and function COLDHOT Low activity High activity -cold temperatures inhibit (prevent) enzyme activity -hot temperatures denature enzymes Best temperature Normal enzyme Extreme heat Denature d enzyme p. 35?

pH Acidic –less than 7 Basic (alkaline) – greater than 7 Neutral = 7 Enzymes have a certain pH at which they work best -above or below optimum pH inhibits activity pH = 0pH = 14 p. 35?

How Enzymes Work Biological Catalysis (usually proteins) – Make a reaction faster Enzymes are Proteins – built to work “active site” Proteins are Happy’ist in their natural Temp and pH –Usually ~37 0 C or 98 0 F & Usually a neutral pH (Function) –What would happen if you boil it? Guesses -___________ –What would happen if you put it in acid? ________________ –What would happen in the cold?________________ Enzymes can only work so fast…….. –If you keep adding substrate will the speed of the Rxn Catalase H2O2H2O2 O 2 & H 2 O Catalase or a)b) Poison What does cyanide do? Arsenic?

How Enzymes Work Biological Catalysis (usually proteins) – Make a reaction faster Enzymes are Proteins – built to work “active site” Proteins are Happy’ist in their natural Temp and pH –Usually ~37 0 C or 98 0 F & Usually a neutral pH (Function) –What would happen if you boil it? Guesses -____denature________ –What would happen if you put it in acid? _______denature_________ –What would happen in the cold?________inhibit________ Enzymes can only work so fast…….. –If you keep adding substrate will the speed of the Rxn Catalase H2O2H2O2 O 2 & H 2 O Catalase or a)b) Poison What does cyanide do? Arsenic?