In Vivo Optical Imaging: Human Application? Vassiliy Tsytsarev University of Maryland school of Medicine E-mail: tsytsarev@umaryland.edu In this study, optical-resolution photoacoustic microscopy was used to monitor the somatosensory cortex for vascular responses to direct electrical stimulations through a cranial window.

Glucose Metabolism Capillary Glycogen TCA TCA Yongmei Chen and Raymond A Swanson Journal of Cerebral Blood Flow & Metabolism (2003) 23, 137–149; Astrocytes and Brain Injury

Glucose Fluorescence Substitute: 2-NBDG 2-deoxy-d-glucose 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-d-glucopyranose (2-NBDG) D-glucose

Glucose Fluorescence Substitute: In vivo imaging of epileptic activity using 2-NBDG

Epileptic Seizures: Fluorescence Imaging

fMRI vs NIRS

Light Banana

Light Banana: Oxygenation

Main parts of experimental setup

HITACHI: medical application

Spectrum: T-ray

EROS: Optical correlation of the EEG? Evoked Response Optical Signal (EROS) (Gratton et al, 2002, 6, 7)

T-ray imaging

Acknowledgments Drs. Shigeru Tanaka, Hidenao Fukuyama, Kazuyuki Imamura, Ayako Ajima, Hisayuki Ojima, Minoru Kimura and Jerom Ribot – Brain Science Institute of RIKEN and Kyoto University, Japan Dr. Sonya Bahar, Director, Center for Neurodynamics, University of Missouri at St. Louis Daisuke Takeshita and Douglas Joseph Brumm – Ph.D students, Center for Neurodynamics, University of Missouri at St. Louis Song Hu, Junjie Yao, Li Li – Ph.D. student of the Washington University in St. Louis Drs Konstantin Maslov and Lihong Wang – Department of Biomedical Engineering, Washington University in St. Louis

Thank you very much for your attention