OPTOFLUIDIC DEVICES FOR SINGLE CELL MANIPULATION Ana Rita Ribeiro, Ariel Guerreiro, Pedro Jorge New Challenges in the European Area - Young Scientist's.

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Presentation transcript:

OPTOFLUIDIC DEVICES FOR SINGLE CELL MANIPULATION Ana Rita Ribeiro, Ariel Guerreiro, Pedro Jorge New Challenges in the European Area - Young Scientist's 1st International Baku Forum

Outline Introduction Main Principles Computational Model and Results Experimental Preliminary Results Conclusions and Future Work Young Scientist's 1st International Baku Forum2

What are optical tweezers? Young Scientist's 1st International Baku Forum3 Motivation Manipulation: Cell/particle manipulation withouth physical contact; Miniaturization: Diagnostic devices (in vivo cells,...) Main Goals Development of fiber optical tweezers; Study of the forces: Numerical models; Design and optimization of fiber structures; Fabrication of devices; Field of application: Manipulation of Red Blood Cells. Liu, Z. et al Opt. Express, OSA, 2006, 14,

Main Principles Young Scientist's 1st International Baku Forum4 Ashkin, A., Dziedzic, J. M., Bjorkholm, J.E. and Chu, S. (1986), Opt Lett., 11, ; Ashkin, A. (1970), Phys. Rev. Lett., 24, Particle placed in the focusing region of a laser beam: Force  transference of momentum on the particle surface (reflections/refractions). Scatering Force Gradient Force

Computational model and Results Young Scientist's 1st International Baku Forum5 Lorentz Force (dipoles): R. S. RODRIGUES RIBEIRO et al, “Computational Models for New Fiber Optic Tweezers”, Photonic Sensors

Results Young Scientist's 1st International Baku Forum6

Preliminary Results Lens – polimer: – radius ~ μm; Laser Diode 980 nm, P = 100mW; Polystyrene particles: Young Scientist's 1st International Baku Forum7 Experimental Preliminary Results R. S. Rodrigues Ribeiro, et al., New theoretical and experimental methods for the design of fiber optic tweezers, EWOFS, Poland,2013

Conclusion and Future Work Development of a 2D numerical model to calculate optical forces acting on a particle/cell; Fabrication of lenses in optical fibers; Implementation of a 3D numerical model; Study of distinct fiber geometries; Manipulation of RBC Young Scientist's 1st International Baku Forum8

Thanks! Young Scientist's 1st International Baku Forum9