class summary and homework for February 2 since we missed 2 consecutive classes, this class consisted of a review and going over the 2 pending homework assignments –in class hands on alignment of mRNA and genomic sequence using UCSC BLAT –identifying signals in the alignment initiator methionine splice site –scavenger hunt homework

homework due next class (page 1) redo the exercise that we did in class using UCSC BLAT –each person on his or her own (not a team effort) –pick a different chromosome and gene –document the following by including a screen shot of each feature –this should be turned in to Kristen electronically and will be graded –this will really be graded for content, not just for effort –since Kristen will need to grade a lot of these, your grade will be lower if the format of your work makes it difficult to grade specify chromosome and gene provide focused screen shot documenting each feature, clearly labeled if needed, additional brief comment on unusual conditions: –big deviaiton from expected signal –no polyA signal found

homework (page 2) –identify and document initiator methionine coding for first three amino acids splice donor splice acceptor stop codon polyA signal

homework (page 3) group projects –try to focus on 3 or 4 possibilities –we will spend some class time trying to define a specific starting point for each group project –some roles for team members programming testing organization, management,scheduling documentation user manual design of user interface (UI)

homework (page 4) readings on The New Biology Lab, PCR and sequencing – imer/genetics_molecular.htmlhttp:// imer/genetics_molecular.html read the whole thing focus especially on: –PCR –DNA sequencing –chapter 6 in Strachan and Reid (NCBI online books) –if this is too much, at least read the PCR and DNA sequencing part in the primer/genetics_molecular.html