Running GC

Running GC Launch the “TotalChrom” Software Click on “Run” Select “Take Control…” Select “Clarus 580” Click “OK”

Running GC The GC touch screen panel should show that the GC is in external control. Once in external control: Build Method Build Sequence

Build Method

Build Method Click on “Method” button

Build Method Select “Create new method” Click on “OK”

Build Method Type in a description Click “Next” Type in a header text The Data Acquisition window should look like the following Click “Next”

Build Method Autosampler tab should look like the following Adjust as you see fit What is shown is a good standard Click “Oven/Inlets” Tab

Build Method Adjust the Oven Ramp program as directed. Make sure to hit enter on the keyboard after every entry Adjust the “Injector A” temperature. Typically this is set around 225 C. Click on “Carrier” Tab

Build Method Adjust the Carrier tab as directed. Typical settings are shown. Click on “Detectors” Tab

Build Method Adjust the Detectors tab as directed. Typical settings are shown. Click on “Next”

Build Method (No Need to Mess with) Click “Next” Click “Next” Click “Finish” Click “Next”

Build Method File  Save Create a unique name Click on “Save” Close Method window

Build Sequence

Build Sequence Click on “Sequence” button

Build Sequence Select “Create new sequence” Click on “OK”

Build Sequence Select the listed options Click on “OK”

Build Sequence Type in a unique name Type in which vial to be used Select a method (brings up a new window) Click on “Add+Close” if all vials are added or click on “Add” to add more vials

Build Sequence File  Save Create a unique name Click on “Save” Close Sequence window

Running Sequence

Running Sequence Click on “Setup” button

Running Sequence Select a sequence (new window will open / not shown) Click OK

Running Sequence The GC will take some time to upload the sequence information, equilibrate, and do pre-run operation You will know that the sequence upload properly because the Instrument-Hands On and Instrument-Modify will no longer be grayed out Once the everything is ready the lettering here should be in green. I/F will read Ready Click on Run Start Run