Do Now— Turn your Do Now into the front What grade do you think you earned on your final? Why? Why grade do you think you earned on the EOC (on a score from 1-9)?
Ms. C’s Absence Hi, I’ve been admitted to the hospital. My wound hasn’t healed and the doctor saw the hardware this morning (not good). I’m going to have surgery to have the hardware removed and get IV antibiotics. I’ll be back when I can. Grades are as up to date as possible.
Objective SWBAT analyze DNA profiles created by gel electrophoresis. SWBAT practice micropipetting and loading gels.
DNA Profiling / DNA Fingerprinting ent/labs/gel/ ent/labs/gel/
PCR The polymerase chain reaction (PCR) is used to amplify specific regions of the DNA. The process uses the same enzymes used by cells to replicate DNA and short sections of DNA that act as primers
PCR Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
Restriction Fragment Length Polymorphism Restriction enzymes cut DNA at specific spots that are nucleotide sequence-specific. The different cutting pattern will lead to differently sized products that can be separated in gel electrophoresis.
Gel Electrophoresis
DNA is negatively charged Smaller DNA fragments travel further during electrophoresis.
DNA Profiling Paternity Testing: Children inherit half of their alleles from each parent and thus should possess a combination of their parents alleles
DNA Profiling Paternity Testing: Children inherit half of their alleles from each parent and thus should possess a combination of their parents alleles
DNA Profiling Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
DNA Profiling Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
DNA Profiling Forensic Investigation: Suspect DNA should be a complete match with the sample taken from a crime scene if a conviction is to occur
Classwork Complete the worksheet to the best of your ability… *This worksheet shows single stranded DNA *This restriction enzyme makes a blunt cut
Worksheet Standard CCATCCAAGACATTATGCAGG/CCTAGACTATT ACGGCCATACCAAGG/CCCACTGG/CCAAACAC ACCCATCAGG/CCATGG/CC CCATCCAAGACATTATGCAGGCCTAGACTATTA CGGCCATACCAAGGCCCACTGGCCAAACAC ACCCATCAGGCCATGGCC 21/26/8/18/6/2 Then color the boxes on the sheet that correspond *This worksheet shows single stranded DNA *This restriction enzyme makes a blunt cut
Classwork *This worksheet shows double stranded DNA as it should be *This restriction enzyme makes a sticky cut
Worksheet Ear Phone DNA 6/23/15/3 Then color the boxes on the sheet that correspond GTCGACCGGTGACCGTGCGTACAGTGCTAT CAGCTGGCCACTGGCACGCATGTCACGATA CCGGATAGCTAATAGCTCCGGTG GGCCTATCGATTATCGAGGCCAC *This worksheet shows double stranded DNA as it should be *This restriction enzyme makes a sticky cut GTCGAC CGGTGACCGTGCGTACAGTGCTAT CAGCTGGC CACTGGCACGCATGTCACGATA C CGGATAGCTAATAGCTC CGGTG GGC CTATCGATTATCGAGGC CAC *You count the pairs